Citation: Zixiang Yuan, Jun Chen, Qin Zhou, Ao Liu, Zeming Qiang, Min Fang, Man Chen, Yan Feng, Haizhu Yu, Xiuli Yang, Xiangming Meng. A lysosomal polarity-specific two-photon fluorescent probe for visualization of autophagy[J]. Chinese Chemical Letters, ;2021, 32(5): 1803-1808. doi: 10.1016/j.cclet.2020.12.012 shu

A lysosomal polarity-specific two-photon fluorescent probe for visualization of autophagy

Zixiang Yuan ^{a, 1} ,
Jun Chen ^{a, 1} ,
Qin Zhou ^{c, 1} ,
Ao Liu ^a ,
Zeming Qiang ^d ,
Min Fang ^a ,
Man Chen ^a ,
Yan Feng ^{a, **,} ,
Haizhu Yu ^{a, **,} ,
Xiuli Yang ^{b, **,} ,
Xiangming Meng ^{a, c, *,}

a.
School of Chemistry and Chemical Engineering & Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei 230601, China
b.
Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province, Yancheng Institute of Technology, Yancheng 224051, China
c.
Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China
d.
Anhui Golden Sun Biochemical Pharmaceuticals Limited Company, Fuyang 236000, China

Received Date: 14 September 2020
Revised Date: 4 December 2020
Accepted Date: 7 December 2020
Available Online: 11 December 2020

Figures(6)

Autophagy plays a vital role in maintaining the balance of normal physiological state of living cells. In this paper, a polarity-specific two-photon fluorescent probe Lyso-NA based on naphthalimide was synthesized for the purpose of monitoring autophagy during biological research. The results of photophysical properties and theoretical calculation confirmed that different polarities of solvents mainly effected fluorescent intensities of probe. Fluorescent intensity, quantum yield and fluorescence lifetime of probe kept a good linear relationship with polarity respectively. In addition, due to its low toxicity and selective accumulation in lysosomes, Lyso-NA is suitable for detecting changes in lysosomal polarity of living cells. Compare with the imaging results of plasmid transfection, a better performed real-time long-term fluorescent visualization of autophagy in living cells was achieved. Probe Lyso-NA can work as an efficient and cost effective imaging tool for visualizing autophagy in living cells.
- Two-photon fluorescent probe,
- Lysosomal polarity,
- Autophagy,
- Visualization,
- Plasmid transfection
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