Citation: Wenmei Zhang, Zunsheng Han, Yingqi Liang, Qi Zhang, Xiangnan Dou, Guangsheng Guo, Xiayan Wang. A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells[J]. Chinese Chemical Letters, ;2021, 32(7): 2183-2186. doi: 10.1016/j.cclet.2020.12.007 shu

A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells

a.
Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, China
b.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

xiayanwang@bjut.edu.cn

Received Date: 9 October 2020
Revised Date: 11 November 2020
Accepted Date: 3 December 2020
Available Online: 11 December 2020

Figures(4)

MicroRNAs are a class of important biomarkers, and the simultaneous detection of multiple miRNAs can provide valuable information about many diseases and biological processes. Amplification-free determination has been developed for the analysis of multiple miRNAs because of its characteristic low cost and high fidelity. Herein, a method for the amplification-free analysis and simultaneous detection of multiple miRNAs based on a so-called pico-HPLC-LIF system is described. In this process, a bare open capillary with an inner diameter of 680 nm is used as a separation column for a sample volume of several hundreds of femtoliters (300 fL), followed by separation and detection. The technique has a zeptomolar limit of detection. The method was applied to detect cellular miRNA from adenocarcinomic human alveolar basal epithelial (A549) cell extracts, and the simultaneous detection of the mir-182, miR-155, and let-7a was achieved. The results showed that the expression of mir-182 and miR-155 was up-regulated and that of let-7a was down-regulated in A549 cells. This method for multiple miRNAs detection is expected to have broad applications in miRNA-based disease diagnosis, prognosis, treatment, and monitoring.
- Multiple miRNAs,
- Amplification-free,
- Pico-HPLC,
- Nanocapillary,
- Laser-induced fluorescence
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1.
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