Citation: Xiaoqi Tao, Ziyi Liao, Yaqing Zhang, Fei Fu, Mengqi Hao, Yang Song, Erqun Song. Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus[J]. Chinese Chemical Letters, ;2021, 32(2): 791-795. doi: 10.1016/j.cclet.2020.07.020 shu

Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus

Xiaoqi Tao ^b ,
Ziyi Liao ^a ,
Yaqing Zhang ^b ,
Fei Fu ^a ,
Mengqi Hao ^a ,
Yang Song ^a ,
Erqun Song ^{a, *,}

a.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
b.
College of Food Science, Southwest University, Chongqing 400715, China

eqsong@swu.edu.cn

Received Date: 27 April 2020
Revised Date: 4 June 2020
Accepted Date: 8 July 2020
Available Online: 9 July 2020

Figures(5)

The detection of bacterial pathogen such as Staphylococcus aureus (S. aureus) is essential for the regulation of food hygiene and disease diagnosis. Herein, we developed a simple one-step fluorescence resonance energy transfer (FRET)-based sensor for specific and sensitive detection of S. aureus in food and serum samples, in which aptamer-modified quantum dots (aptamer-QDs) was employed as the energy donor and antibiotic of teicoplanin functionalized-gold nanoparticles (Teico-AuNPs) was chosen as the energy acceptor. Within 1 h, the FRET-based sensor showed a linear range of from 10 cfu/mL to 5×10⁸ cfu/mL, with the low limit of detection (LOD, 2 cfu/mL) for S. aureus in buffer. When further applied to assay S. aureus in real samples, the FRET-based sensor showed good recoveries ranging from 84.5% to 110.0%, with relative standard derivations (RSDs) of 0.01%–0.44% and a LOD of 100 cfu/mL in milk, orange juice and human serum.
- Fluorescence resonance energy transfer,
- (FRET),
- Staphylococcus aureus,
- Aptamer,
- Quantum dot,
- Teicoplanin,
- Gold nanoparticles
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