Citation: Jiafu Chang, Wenxin Lv, Jiahui Wu, Haiyin Li, Feng Li. Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification[J]. Chinese Chemical Letters, ;2021, 32(2): 775-778. doi: 10.1016/j.cclet.2020.05.041 shu

Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification

Jiafu Chang ^{a, b} ,
Wenxin Lv ^a ,
Jiahui Wu ^a ,
Haiyin Li ^{a, *,} ,
Feng Li ^{a, b, *,}

a.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
b.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, China

lihaiyin@qau.edu.cn

lifeng@qau.edu.cn

Received Date: 15 April 2020
Revised Date: 19 May 2020
Accepted Date: 28 May 2020
Available Online: 31 May 2020

Figures(4)

Herein, we propose a novel photoelectrochemical (PEC) biosensor for dual microRNAs (miRNAs) highly sensitive and simultaneous biosensing based on strand displaced amplification (SDA) reaction. The recognition of HmiR-21 and Hlet-7a by microRNA-21 and let-7a leads to their change in hairpin structures, subsequently initiating the immobilization of abundant CdS quantum dots (CdS QDs) and methylene blue (MB) based on SDA reaction. The immobilized CdS QDs and MB produce both high PEC currents under 430 nm light and 627 nm light illumination, respectively, and the generated PEC currents are closely relied on target miRNAs amounts. Thus, highly sensitive and simultaneous detection of microRNA-21 Herein, we propose a novel photoelectrochemical (PEC) biosensor for dual microRNAs (miRNAs) highly sensitive and simultaneous biosensing based on strand displaced amplification (SDA) reaction. The recognition of H_miR-21 and H_let-7a by microRNA-21 and let-7a leads to their change in hairpin structures, subsequently initiating the immobilization of abundant CdS quantum dots (CdS QDs) and methylene blue (MB) based on SDA reaction. The immobilized CdS QDs and MB produce both high PEC currents under 430 nm light and 627 nm light illumination, respectively, and the generated PEC currents are closely relied on target miRNAs amounts. Thus, highly sensitive and simultaneous detection of microRNA-21 and let-7a was readily achieved with detection limit at 6.6 fmol/L and 15.4 fmol/L based on 3σ, respectively. Further, this PEC biosensor was applied in simultaneous analysis of miRNA-21 and let-7a in breast cancer patient's serum with acceptable results. We expect this biosensor will find more useful application in diagnosis of miRNA-related diseases.
- PEC,
- CdS QDs,
- Methylene blue,
- Multiple microRNAs,
- Strand displaced amplification,
- Simultaneous biosensing
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