Citation: Liu Haifang, Sun Yuanqiang, Li Zhaohui, Yang Ran, Yang Jie, Aryee Aaron Albert, Zhang Xiaoge, Ge Jia, Qu Lingbo, Lin Yuehe. SciFinder-guided rational design of fluorescent carbon dots for ratiometric monitoring intracellular pH fluctuations under heat shock[J]. Chinese Chemical Letters, ;2019, 30(9): 1647-1651. doi: 10.1016/j.cclet.2019.06.012 shu

SciFinder-guided rational design of fluorescent carbon dots for ratiometric monitoring intracellular pH fluctuations under heat shock

Liu Haifang ^a,1 ,
Sun Yuanqiang ^a,1 ,
Li Zhaohui ^a,*, ,
Yang Ran ^a ,
Yang Jie ^a ,
Aryee Aaron Albert ^a ,
Zhang Xiaoge ^a,*, ,
Ge Jia ^a ,
Qu Lingbo ^a,*, ,
Lin Yuehe ^b

a.
Institute of Chemical Biology and Clinical Application at The First Affiliated Hospital, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China
b.
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States

zhaohui.li@zzu.edu.cn

zhangxiaogege@126.com

qulingbo@zzu.edu.cn

Received Date: 13 May 2019
Revised Date: 5 June 2019
Accepted Date: 5 June 2019
Available Online: 6 September 2019

Figures(6)

Intracellular pH plays a significant role in various biological processes, including cell proliferation, apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation of pH responsive carbon dots (CDs-pH) for ratiometric intracellular imaging was reported. By using SciFinder database, fluorescent CDs-pH with the required pK_a value of 6.84 were rationally designed, which is vital important for precise sensing of intracellular pH. As a result, the synthesized CDspH demonstrated robust ability to test pH fluctuations within the physiological range of 5.4-7.4. The CDspH was further utilized for fluorescent ratiometric imaging of pH in living HeLa cells, effectively avoided the influence of autofluorescence from native cellular species. Moreover, real-time monitoring of intracellular pH fluctuation under heat shock was successfully realized. This SciFinder-guided design strategy is simple and flexible, which has a great potential to be used for the development of other types of CDs for various applications.
- Carbon dots,
- Rationally design,
- SciFinder database,
- Ratiometric monitoring,
- Intracellular pH
1. [1]
  J.T. Hou, W.X. Ren, K. Li, et al., Chem. Soc. Rev. 46(2017) 2076-2090. doi: 10.1039/C6CS00719H
2. [2]
  K.A. White, B.K. Grillo-Hill, D.L. Barber, J. Cell. Sci. 130(2017) 663-669. doi: 10.1242/jcs.195297
3. [3]
  C. Stock, S.F. Pedersen, Semin. Cancer Biol. 43(2017) 5-16. doi: 10.1016/j.semcancer.2016.12.001
4. [4]
  T.M. Wang, L.C. Holzhausen, R.H. Kramer, Nat. Neurosci. 17(2014) 262-270. doi: 10.1038/nn.3627
5. [5]
  B.A. Webb, M. Chimenti, M.P. Jacobson, D.L. Barber, Nat. Rev. Cancer 11(2011) 671-677. doi: 10.1038/nrc3110
6. [6]
  S. Chen, Y. Hong, Y. Liu, et al., J. Am. Chem. Soc. 135(2013) 4926-4929. doi: 10.1021/ja400337p
7. [7]
  Y.T. Tsai, J. Zhou, H. Weng, et al., Adv. Healthcare Mater. 3(2014) 221-229. doi: 10.1002/adhm.201200365
8. [8]
  S.K.A. Opare, A. Petoyan, B. Mehrazma, A. Rauk, Can. J. Chem. 94(2016) 273-281.
9. [9]
  S. Kobayashi, Y. Tanaka, M. Kiyono, et al., J. Mol. Struct. 1094(2015) 109-117. doi: 10.1016/j.molstruc.2015.03.023
10. [10]
  T. Yang, Y.K. Li, M.L. C, J.H. Wang, Chin. Chem. Lett. 26(2015) 1496-1501. doi: 10.1016/j.cclet.2015.10.018
11. [11]
  J. Zhang, S. Wu, X. Lu, P. Wu, J. Liu, Nano Lett. 19(2019) 3214-3220. doi: 10.1021/acs.nanolett.9b00725
12. [12]
  Q.Y. Cai, J. Li, J. Ge, et al., Biosens. Bioelectron. 72(2015) 31-36. doi: 10.1016/j.bios.2015.04.077
13. [13]
  J. Xia, S. Chen, G.Y. Zou, Y.L. Yu, J.H. Wang, Nanoscale 10(2018) 22484-22492. doi: 10.1039/C8NR08208A
14. [14]
  S. E, Q.X. Mao, X.L. Yuan, et al., Nanoscale 10(2018) 12788-12796. doi: 10.1039/C8NR03453B
15. [15]
  W. Shi, X. Li, H. Ma, Angew. Chem. Int. Ed. 124(2012) 6538-6541. doi: 10.1002/ange.201202533
16. [16]
  H. Zhang, B. Zhang, C. Di, et al., Nanoscale 10(2018) 5342-5349. doi: 10.1039/C7NR08987B
17. [17]
  Z. Li, Y. Wang, J. Wang, et al., Anal. Chem. 82(2010) 7008-7014. doi: 10.1021/ac101405a
18. [18]
  L. Yuan, W. Lin, K. Zheng, L. He, W. Huang, Chem. Soc. Rev. 42(2013) 622-661. doi: 10.1039/C2CS35313J
19. [19]
  R. Strack, Nat. Methods 12(2015) 1111. doi: 10.1038/nmeth.3676
20. [20]
  N. Boens, V. Leen, W. Dehaen, Chem. Soc. Rev. 41(2012) 1130-1172. doi: 10.1039/C1CS15132K
21. [21]
  M. Vendrell, D. Zhai, J.C. Er, Y.T. Chang, Chem. Rev. 112(2012) 4391-4420. doi: 10.1021/cr200355j
22. [22]
  S. Sun, Q. Guan, Y. Liu, et al., Chin. Chem. Lett. 30(2019) 1051-1054. doi: 10.1016/j.cclet.2019.01.014
23. [23]
  H. Liu, Y. Sun, J. Yang, et al., Sens. Actuators B-Chem. 280(2019) 62-68. doi: 10.1016/j.snb.2018.10.029
24. [24]
  K. Jiang, S. Sun, L. Zhang, et al., Angew. Chem. Int. Ed. 54(2015) 5360-5363. doi: 10.1002/anie.201501193
25. [25]
  H. Liu, Y. Sun, Z. Li, et al., Nanoscale 11(2019) 8458-8463. doi: 10.1039/C9NR01678C
26. [26]
  S. Lu, L. Sui, J. Liu, et al., Adv. Mater. 29(2017) 1603443-1603448. doi: 10.1002/adma.201603443
27. [27]
  D. Rodríguez-Padrón, A.D. Jodlowski, G. de Miguel, et al., Green Chem. 20(2018) 225-229. doi: 10.1039/C7GC03295A
28. [28]
  D. Heine, S. Sundaram, M. Beudert, K. Martin, C. Hertweck, Chem. Sci. 7(2016) 4848-4855. doi: 10.1039/C6SC00503A
29. [29]
  X. Li, X. Gao, W. Shi, H. Ma, Chem. Rev. 114(2014) 590-659. doi: 10.1021/cr300508p
30. [30]
  Y.F. Kang, Y.W. Fang, Y.H. Li, W. Li, X.B. Yin, Chem. Commun. 51(2015) 16956-16959. doi: 10.1039/C5CC06304C
31. [31]
  S. Ruan, J. Qian, S. Shen, et al., Nanoscale 6(2014) 10040-10047. doi: 10.1039/C4NR02657H
32. [32]
  R.S. Li, P.F. Gao, H.Z. Zhang, et al., Chem. Sci. 8(2017) 6829-6835. doi: 10.1039/C7SC01316G
33. [33]
  H. Liu, Z. Li, Y. Sun, et al., Sci. Rep. 8(2018) 1086. doi: 10.1038/s41598-018-19373-3
34. [34]
  L. Lin, J. Zhao, L. Zhang, et al., Chem. Commun. 54(2018) 9071-9074. doi: 10.1039/C8CC05385E
35. [35]
  Q. Wan, S. Chen, W. Shi, L. Li, H. Ma, Angew. Chem. Int. Ed. 126(2014) 11096-11100. doi: 10.1002/ange.201405742
36. [36]
  W. Xu, Z. Zeng, J.H. Jiang, Y.T. Chang, L. Yuan, Angew. Chem. Int. Ed. 55(2016) 13658-13699. doi: 10.1002/anie.201510721
1. [1]
  Meiling Xu , Xinyang Li , Pengyuan Liu , Junjun Liu , Xiao Han , Guodong Chai , Shuangling Zhong , Bai Yang , Liying Cui . A novel and visible ratiometric fluorescence determination of carbaryl based on red emissive carbon dots by a solvent-free method. Chinese Chemical Letters, 2025, 36(2): 109860-. doi: 10.1016/j.cclet.2024.109860
2. [2]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
3. [3]
  Qiang Fu , Shouhong Sun , Kangzhi Lu , Ning Li , Zhanhua Dong . Boron-doped carbon dots: Doping strategies, performance effects, and applications. Chinese Chemical Letters, 2024, 35(7): 109136-. doi: 10.1016/j.cclet.2023.109136
4. [4]
  Chenghao Liu , Xiaofeng Lin , Jing Liao , Min Yang , Min Jiang , Yue Huang , Zhizhi Du , Lina Chen , Sanjun Fan , Qitong Huang . Carbon dots-based dopamine sensors: Recent advances and challenges. Chinese Chemical Letters, 2024, 35(12): 109598-. doi: 10.1016/j.cclet.2024.109598
5. [5]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
6. [6]
  Yuan Liu , Boyang Wang , Yaxin Li , Weidong Li , Siyu Lu . Understanding excitonic behavior and electroluminescence light emitting diode application of carbon dots. Chinese Chemical Letters, 2025, 36(2): 110426-. doi: 10.1016/j.cclet.2024.110426
7. [7]
  Rui Cheng , Tingting Zhang , Xin Huang , Jian Yu . Facile synthesis of high-brightness green-emitting carbon dots with narrow bandwidth towards backlight display. Chinese Chemical Letters, 2024, 35(5): 108763-. doi: 10.1016/j.cclet.2023.108763
8. [8]
  Wu-Jian Long , Yang Yu , Chuang He . A novel and promising engineering application of carbon dots: Enhancing the chloride binding performance of cement. Chinese Chemical Letters, 2024, 35(6): 108943-. doi: 10.1016/j.cclet.2023.108943
9. [9]
  Qiang Li , Jiangbo Fan , Hongkai Mu , Lin Chen , Yongzhen Yang , Shiping Yu . Nucleus-targeting orange-emissive carbon dots delivery adriamycin for enhanced anti-liver cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108947-. doi: 10.1016/j.cclet.2023.108947
10. [10]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
11. [11]
  Xiaoning Li , Quanyu Shi , Meng Li , Ningxin Song , Yumeng Xiao , Huining Xiao , Tony D. James , Lei Feng . Functionalization of cellulose carbon dots with different elements (N, B and S) for mercury ion detection and anti-counterfeit applications. Chinese Chemical Letters, 2024, 35(7): 109021-. doi: 10.1016/j.cclet.2023.109021
12. [12]
  Hao Cai , Xiaoyan Wu , Lei Jiang , Feng Yu , Yuxiang Yang , Yan Li , Xian Zhang , Jian Liu , Zijian Li , Hong Bi . Lysosome-targeted carbon dots with a light-controlled nitric oxide releasing property for enhanced photodynamic therapy. Chinese Chemical Letters, 2024, 35(4): 108946-. doi: 10.1016/j.cclet.2023.108946
13. [13]
  Liwen Wang , Boyang Wang , Siyu Lu , Shubo Lv , Xiaoli Qu . High quantum yield yellow emission carbon dots for the construction of blue light blocking films. Chinese Chemical Letters, 2025, 36(2): 110497-. doi: 10.1016/j.cclet.2024.110497
14. [14]
  Rui Cheng , Xin Huang , Tingting Zhang , Jiazhuang Guo , Jian Yu , Su Chen . Solid superacid catalysts promote high-performance carbon dots with narrow-band fluorescence emission for luminescence solar concentrators. Chinese Chemical Letters, 2024, 35(8): 109278-. doi: 10.1016/j.cclet.2023.109278
15. [15]
  Xiuzheng Deng , Changhai Liu , Xiaotong Yan , Jingshan Fan , Qian Liang , Zhongyu Li . Carbon dots anchored NiAl-LDH@In₂O₃ hierarchical nanotubes for promoting selective CO₂ photoreduction into CH₄. Chinese Chemical Letters, 2024, 35(6): 108942-. doi: 10.1016/j.cclet.2023.108942
16. [16]
  Binyang Qin , Mengqi Wang , Shimei Wu , Yining Li , Chilin Liu , Yufei Zhang , Haosen Fan . Carbon dots confined nanosheets assembled NiCo₂S₄@CDs cross-stacked architecture for enhanced sodium ion storage. Chinese Chemical Letters, 2024, 35(7): 108921-. doi: 10.1016/j.cclet.2023.108921
17. [17]
  Chaoqun Ma , Yuebo Wang , Ning Han , Rongzhen Zhang , Hui Liu , Xiaofeng Sun , Lingbao Xing . Carbon dot-based artificial light-harvesting systems with sequential energy transfer and white light emission for photocatalysis. Chinese Chemical Letters, 2024, 35(4): 108632-. doi: 10.1016/j.cclet.2023.108632
18. [18]
  Shuangying Li , Qingxiang Zhou , Zhi Li , Menghua Liu , Yanhui Li . Sensitive measurement of silver ions in environmental water samples integrating magnetic ion-imprinted solid phase extraction and carbon dot fluorescent sensor. Chinese Chemical Letters, 2024, 35(5): 108693-. doi: 10.1016/j.cclet.2023.108693
19. [19]
  Xiuzheng Deng , Yi Ke , Jiawen Ding , Yingtang Zhou , Hui Huang , Qian Liang , Zhenhui Kang . Construction of ZnO@CDs@Co₃O₄ sandwich heterostructure with multi-interfacial electron-transfer toward enhanced photocatalytic CO₂ reduction. Chinese Chemical Letters, 2024, 35(4): 109064-. doi: 10.1016/j.cclet.2023.109064
20. [20]
  Jie Wu , Xiaoqing Yu , Guoxing Li , Su Chen . Engineering particles towards 3D supraballs-based passive cooling via grafting CDs onto colloidal photonic crystals. Chinese Chemical Letters, 2024, 35(4): 109234-. doi: 10.1016/j.cclet.2023.109234

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(1105)
HTML views(87)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142