Citation: Sun Yanhui, Zhan Aiyan, Zhou Shuang, Kuang Xiao, Shen Hongyan, Liu Hongzhuo, Xu Youjun. A novel mitochondria-targeting tetrapeptide for subcellular delivery of nanoparticles[J]. Chinese Chemical Letters, ;2019, 30(7): 1435-1439. doi: 10.1016/j.cclet.2019.05.001 shu

A novel mitochondria-targeting tetrapeptide for subcellular delivery of nanoparticles

Sun Yanhui ^a ,
Zhan Aiyan ^b ,
Zhou Shuang ^b ,
Kuang Xiao ^b ,
Shen Hongyan ^a ,
Liu Hongzhuo ^b,*, ,
Xu Youjun ^a,*,

a.
School of Pharmaceutial Engineering, Key Laboratory of Structure-Based Drug Design & Discovery(Ministry of Education), Shenyang Pharmaceutical University, Shenyang 110016, China
b.
Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China

liuhongzhuo@syphu.edu.cn

xuyoujun@syphu.edu.cn

Received Date: 16 March 2019
Revised Date: 19 April 2019
Accepted Date: 5 May 2019
Available Online: 7 July 2019

Figures(5)

Mitochondrial dysfunction is associated with the emergence of several neurological and cardiovascular diseases. Hence, mitochondria-targeting delivery strategies are highly significant and critically needed. In this study, we developed a small library of peptides simulating the mitochondria-targeting peptide SS-31, a promising tetra-peptide with antioxidant character, and subsequently evaluated the toxicity, antioxidant ability and mitochondrial delivery of nanoparticles. Among the designed peptides, RF-2 (DArg-Dmt-Arg-Phe-NH₂) showed controlled toxicity and excellent protection against gentamicin-induced hair cell damage, as compared with SS-31. More importantly, RF-2-modified PLGA nanoparticles demonstrated high colocalization with mitochondria and comparable specific subcellular accumulation, when compared with SS-31. Taken together, the obtained results supported RF-2 as a mitochondriatargeting peptide with high potential as a targeted carrier.
- Mitochondrial targeting,
- Peptide,
- SS-31,
- Toxicity,
- Nanoparticles,
- Zebrafish
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沈阳化工大学材料科学与工程学院沈阳 110142