Citation: Song Chunxia, Xiao Yuxin, Li Kunpeng, Zhang Xiaoyu, Lu Ying. β-Cyclodextrin polymer based fluorescence enhancement method for sensitive adenosine triphosphate detection[J]. Chinese Chemical Letters, ;2019, 30(6): 1249-1252. doi: 10.1016/j.cclet.2019.03.055 shu

β-Cyclodextrin polymer based fluorescence enhancement method for sensitive adenosine triphosphate detection

Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, China

E-mail addresses:

songchunxia@ahau.edu.cn

luy@ahau.edu.cn

Received Date: 24 January 2019
Revised Date: 22 March 2019
Accepted Date: 29 March 2019
Available Online: 30 June 2019

Figures(6)

Adenosine triphosphate plays a crucial role in regulation of many biological pathways and has been used as an indicator for many diseases. In this paper, based on the fact that β-cyclodextrin polymer (polyβ-CD) could significantly enhance pyrene fluorescence through supramolecular assembly (host-gest interaction), a sensitive and facile method for adenosine triphosphate detection has been developed. A 3'-pyrene-labelled ATP-binding aptamer was employed as the fluorescence probe, which could be digested by exonuclease Ⅰ to obtain mononucleotides, with pyrene attached on. The pyrene attached on mononucleotides could easily enter the hydrophobic cavity of polyβ-CD, accompanied with prominent fluorescence enhancement. While ATP was introduced, ATP and its aptamer could combine together and the obtained hairpin complex could not be cleaved by exonuclease Ⅰ. The pyrene labelled on the probe could not enter the cavity of polyβ-CD belong to the complex' steric hindrance, accompanied with the weak pyrene' fluorescence. So we could quantify the concentration of adenosine triphosphate facilely by measuring the fluorescence intensity of the system. The detection limit of this method for adenosine triphosphate was 11 μmol/L (S/N=3). The developed method showed sufficient selectivity and could successfully assay adenosine triphosphate in biological samples. The developed method provides a potential platform for biological micromoles assay.
- β-Cyclodextrin polymer,
- Supramolecular assembly,
- Fluorescence,
- Enhancement,
- Adenosine triphosphate
1. [1]
  X. Feng, Y. Jia, P. Cai, et al., ACS Nano 10(2016) 556-561. doi: 10.1021/acsnano.5b05579
2. [2]
  D.R. Palleros, K.L. Raid, L. Shi, et al., Nature 365(1993) 664-666. doi: 10.1038/365664a0
3. [3]
  X. Zheng, R. Peng, X. Jiang, et al., Anal. Chem. 89(2017) 10941-10947. doi: 10.1021/acs.analchem.7b02763
4. [4]
  H. Yokoshiki, M. Sunagawa, T. Seki, et al., Am. J. Physiol. 274(1998) C25-C37. doi: 10.1152/ajpcell.1998.274.1.C25
5. [5]
  A.V. Gourine, E. Llaudet, N. Dale, et al., Nature 436(2005) 108-111. doi: 10.1038/nature03690
6. [6]
  L. Annunziato, G. Pignataro, R.G. Di, Pharmacol. Rev. 56(2004) 633-654. doi: 10.1124/pr.56.4.5
7. [7]
  C. Zhang, R.A. Rissman, J. Feng, J. Alzheimers Dis. 44(2015) 375-378. doi: 10.3233/JAD-141890
8. [8]
  X. Zhang, C. Song, K. Yang, et al., Anal. Chem. 90(2018) 4968-4971. doi: 10.1021/acs.analchem.7b05442
9. [9]
  Z. Qing, L. Hou, L. Yang, et al., Anal. Chem. 88(2016) 9759-9765. doi: 10.1021/acs.analchem.6b02720
10. [10]
  L. Yang, Z. Qing, C. Liu, et al., Anal. Chem. 88(2016) 9285-9292. doi: 10.1021/acs.analchem.6b02667
11. [11]
  X. Zhang, R. Kong, Q. Tan, et al., Talanta 169(2017) 1-7. doi: 10.1016/j.talanta.2017.03.050
12. [12]
  Q. Tan, R. Zhang, R. Kong, et al., Microchim. Acta 185(2018) 44-50. doi: 10.1007/s00604-017-2603-7
13. [13]
  D.E. Huizenga, J.W. Szostak, Biochemistry 34(1995) 656-665. doi: 10.1021/bi00002a033
14. [14]
  X.M. Hai, N. Li, K. Wang, et al., Anal. Chim. Acta 998(2018) 60-66. doi: 10.1016/j.aca.2017.10.028
15. [15]
  B. Huang, Z. Geng, S. Yan, et al., Anal. Chem. 89(2017) 8816-8821. doi: 10.1021/acs.analchem.7b01212
16. [16]
  X. Zheng, R. Peng, X. Jiang, et al., Anal. Chem. 89(2017) 10941-10947. doi: 10.1021/acs.analchem.7b02763
17. [17]
  L. Szente, J. Szemán, Anal. Chem. 85(2013) 8024-8030. doi: 10.1021/ac400639y
18. [18]
  A. Harada, Acc. Chem. Res. 34(2001) 456-464. doi: 10.1021/ar000174l
19. [19]
  K. Uekama, F. Hirayama, T. Irie, Chem. Rev. 98(1998) 2045-2076. doi: 10.1021/cr970025p
20. [20]
  A.R. Hedges, Chem. Rev. 98(1998) 2035-2044. doi: 10.1021/cr970014w
21. [21]
  M. Hollas, M.A. Chung, J. Adams, J. Phys. Chem. B 102(1998) 2947-2953. doi: 10.1021/jp9800719
22. [22]
  C.M. Maragos, M. Appell, V. Lippolis, et al., Food Addit. Contam. A 25(2008) 164-171. doi: 10.1080/02652030701564555
23. [23]
  G. Zhilong, Z. Zhujun, Microchim. Acta 126(1997) 325-328. doi: 10.1007/BF01242340
24. [24]
  C. Cucci, A.G. Mignani, C. Dall'Asta, et al., Sens. Actuators B-Chem. 126(2007) 467-472. doi: 10.1016/j.snb.2007.03.036
25. [25]
  Z. Qing, X. He, J. Huang, et al., Anal. Chem. 86(2014) 4934-4939. doi: 10.1021/ac500834g
26. [26]
  J. Sun, S. Wang, F. Gao, Langmuir 32(2016) 12725-12731. doi: 10.1021/acs.langmuir.6b03002
27. [27]
  C. Song, X. Yang, K. Wang, et al., Chem. Commun. 51(2015) 1815-1818. doi: 10.1039/C4CC08991J
28. [28]
  C. Song, X. Yang, K. Wang, et al., Spectrochim. Acta A 156(2016) 131-137. doi: 10.1016/j.saa.2015.12.001
29. [29]
  P. Liu, S. Sun, X. Guo, et al., Anal. Chem. 87(2015) 2665-2671. doi: 10.1021/ac503301q
30. [30]
  M. Shaikh, J. Mohanty, M. Sundararajan, et al., J. Phys. Chem. B 116(2012) 12450-12459. doi: 10.1021/jp3087368
31. [31]
  L. Wang, C. Zhong, P. Xue, et al., J. Org. Chem. 76(2011) 4874-4883. doi: 10.1021/jo2007829
32. [32]
  C. Song, B. Li, X. Yang, et al., Analyst 142(2017) 224-228. doi: 10.1039/C6AN02269C
33. [33]
  J.A. Cruz-Aguado, G. Penner, Anal. Chem. 80(2008) 8853-8855. doi: 10.1021/ac8017058
34. [34]
  J.A. Cruz-Aguado, G. Penner, J. Agric. Food Chem. 56(2008) 10456-10461. doi: 10.1021/jf801957h
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