Citation: Ding Cong, Chen Yage, Li Haozheng, Wang Bingyao, Wei Qi, Tang Huajun, Jia Shaokang, He Zhiyong, Wang Ping, Zhou Xiang. Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne[J]. Chinese Chemical Letters, ;2019, 30(7): 1393-1396. doi: 10.1016/j.cclet.2019.03.046 shu

Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne

Ding Cong ^a,1 ,
Chen Yage ^b,c,1 ,
Li Haozheng ^b,c,1 ,
Wang Bingyao ^a ,
Wei Qi ^a ,
Tang Huajun ^b,c ,
Jia Shaokang ^a ,
He Zhiyong ^a,*, ,
Wang Ping ^b,c ,
Zhou Xiang ^a,*,

a.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of the Ministry of Education, WuhanUniversity, Wuhan 430072, China
b.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
c.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China

hezy@whu.edu.cn

xzhou@whu.edu.cn

Received Date: 26 February 2019
Revised Date: 25 March 2019
Accepted Date: 26 March 2019
Available Online: 27 July 2019

Figures(4)

We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future
- Raman probe,
- SRS,
- Cell imaging,
- Photo-stability feature
1. [1]
  L. Wei, Z. Chen, L. Shi, et al., Nature 544(2017) 465-470. doi: 10.1038/nature22051
2. [2]
  L. Wei, F. Hu, Y. Shen, et al., Nat. Methods 11(2014) 410-412. doi: 10.1038/nmeth.2878
3. [3]
  Z. Zhao, Y. Shen, F. Hu, W. Min, Analyst 142(2017) 4018-4029. doi: 10.1039/C7AN01001J
4. [4]
  C.W. Freudiger, W. Min, B.G. Saar, et al., Science 322(2008) 1857-1861. doi: 10.1126/science.1165758
5. [5]
  H. Lei, Y. Hu, G. Li, Chin. Chem. Lett. 29(2018) 509-512. doi: 10.1016/j.cclet.2017.08.012
6. [6]
  H. Zhang, L. Sun, Y. Zhang, et al., Chin. Chem. Lett. 29(2018) 981-984. doi: 10.1016/j.cclet.2017.10.017
7. [7]
  F. Hu, C. Zeng, R. Long, et al., Nat. Methods 15(2018) 194-200. doi: 10.1038/nmeth.4578
8. [8]
  L. Wei, F. Hu, Z. Chen, Acc. Chem. Res. 49(2016) 1494-1502. doi: 10.1021/acs.accounts.6b00210
9. [9]
  Z. Chen, D.W. Paley, L. Wei, et al., J. Am. Chem. Soc. 136(2014) 8027-8033. doi: 10.1021/ja502706q
10. [10]
  Z.L. Song, Z. Chen, X. Bian, et al., J. Am. Chem. Soc. 136(2014) 13558-13561. doi: 10.1021/ja507368z
11. [11]
  L. Wei, Y. Shen, F. Xu, et al., ACS Chem. Biol. 10(2015) 901-908. doi: 10.1021/cb500787b
12. [12]
  H. Yamakoshi, K. Dodo, M. Okada, et al., J. Am. Chem. Soc. 133(2011) 6102-6105. doi: 10.1021/ja108404p
13. [13]
  F. Hu, Z. Chen, L. Zhang, et al., Angew. Chem. Int. Ed. 54(2015) 9821-9825. doi: 10.1002/anie.201502543
14. [14]
  L. Wei, Y. Yu, Y. Shen, M.C. Wang, W. Min, Proc. Natl. Acad. Sci. U. S. A.110(2013) 11226-11231. doi: 10.1073/pnas.1303768110
15. [15]
  L. Lin, X. Tian, S. Hong, Angew. Chem. Int. Ed. 52(2013) 7266-7271. doi: 10.1002/anie.201301387
16. [16]
  Y. Shen, Z. Zhao, L. Zhang, Proc. Natl. Acad. Sci. U. S. A.114(2017) 13394-13399. doi: 10.1073/pnas.1712555114
17. [17]
  A.C. Johansson, H. Appelqvist, C. Nilsson, et al., Apoptosis 15(2010) 527-540. doi: 10.1007/s10495-009-0452-5
18. [18]
  C. Settembre, A. Fraldi, D.L. Medina, A. Ballabio, Nature Rev. Mol. Cell Biol. 14(2013) 283-296. doi: 10.1038/nrm3565
19. [19]
  H. Xu, D. Ren, Annu. Rev. Physiol. 77(2015) 57-80. doi: 10.1146/annurev-physiol-021014-071649
20. [20]
  F.M. Platt, B. Boland, A.C. van der Spoel, J. Cell Biol. 199(2012) 723-734. doi: 10.1083/jcb.201208152
21. [21]
  B.L. Wei, P.W. Denton, E. O'Neill, et al., J. Virol. 79(2005) 5705-5712. doi: 10.1128/JVI.79.9.5705-5712.2005
22. [22]
  X.J. Cao, L.N. Chen, X. Zhang, et al., Anal. Chim. Acta 920(2016) 86-93. doi: 10.1016/j.aca.2016.03.029
23. [23]
  M. Li, H. Ge, V. Mirabello, et al., Chem. Commun. (Camb.) 53(2017) 11161-11164. doi: 10.1039/C7CC05166B
24. [24]
  X.L. Shi, G.J. Mao, X.B. Zhang, et al., Talanta 130(2014) 356-362. doi: 10.1016/j.talanta.2014.07.030
25. [25]
  X. Zhou, F. Su, H. Lu, et al., Biomaterials 33(2012) 171-180. doi: 10.1016/j.biomaterials.2011.09.053
26. [26]
  J.A. Mindell, Annu. Rev. Physiol. 74(2012) 69-86. doi: 10.1146/annurev-physiol-012110-142317
27. [27]
  H. Yamakoshi, K. Dodo, A. Palonpon, et al., J. Am. Chem. Soc. 134(2012) 20681-20689. doi: 10.1021/ja308529n
28. [28]
  J. Zhang, S. Yan, Z. He, et al., J. Phys. Chem. Lett. 9(2018) 4679-4685. doi: 10.1021/acs.jpclett.8b01991
1. [1]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
2. [2]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
3. [3]
  Zhixue Liu , Haiqi Chen , Lijuan Guo , Xinyao Sun , Zhi-Yuan Zhang , Junyi Chen , Ming Dong , Chunju Li . Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging. Chinese Chemical Letters, 2024, 35(9): 109666-. doi: 10.1016/j.cclet.2024.109666
4. [4]
  Qian Ren , Xue Dai , Ran Cen , Yang Luo , Mingyang Li , Ziyun Zhang , Qinghong Bai , Zhu Tao , Xin Xiao . A cucurbit[8]uril-based supramolecular phosphorescent assembly: Cell imaging and sensing of amino acids in aqueous solution. Chinese Chemical Letters, 2024, 35(12): 110022-. doi: 10.1016/j.cclet.2024.110022
5. [5]
  Jia-Mei Qin , Xue Li , Wei Lang , Fu-Hao Zhang , Qian-Yong Cao . An AIEgen nano-assembly for simultaneous detection of ATP and H₂S. Chinese Chemical Letters, 2024, 35(6): 108925-. doi: 10.1016/j.cclet.2023.108925
6. [6]
  Brandon Bishop , Shaofeng Huang , Hongxuan Chen , Haijia Yu , Hai Long , Jingshi Shen , Wei Zhang . Artificial transmembrane channel constructed from shape-persistent covalent organic molecular cages capable of ion and small molecule transport. Chinese Chemical Letters, 2024, 35(11): 109966-. doi: 10.1016/j.cclet.2024.109966
7. [7]
  Xu Qu , Pengzhao Wu , Kaixuan Duan , Guangwei Wang , Liang-Liang Gao , Yuan Guo , Jianjian Zhang , Donglei Shi . Self-calibrating probes constructed on a unique dual-emissive fluorescence platform for the precise tracking of cellular senescence. Chinese Chemical Letters, 2024, 35(12): 109681-. doi: 10.1016/j.cclet.2024.109681
8. [8]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
9. [9]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
10. [10]
  Zhihui Zhang , Ru Sun , Chong Bian , Hongbo Wang , Zhen Zhao , Panpan Lv , Jianzhong Lu , Haixin Zhang , Hulie Zeng , Yuanyuan Chen , Zhijuan Cao . A dual-protease-triggered chemiluminescent probe for precise tumor imaging. Chinese Chemical Letters, 2025, 36(2): 109784-. doi: 10.1016/j.cclet.2024.109784
11. [11]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020
12. [12]
  Jianqiu Li , Yi Zhang , Songen Liu , Jie Niu , Rong Zhang , Yong Chen , Yu Liu . Cucurbit[8]uril-based non-covalent heterodimer realized NIR cell imaging through topological transformation from nanowire to nanorod. Chinese Chemical Letters, 2024, 35(10): 109645-. doi: 10.1016/j.cclet.2024.109645
13. [13]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
14. [14]
  Ce Liang , Qiuhui Sun , Adel Al-Salihy , Mengxin Chen , Ping Xu . Recent advances in crystal phase induced surface-enhanced Raman scattering. Chinese Chemical Letters, 2024, 35(9): 109306-. doi: 10.1016/j.cclet.2023.109306
15. [15]
  Chengde Wang , Liping Huang , Shanshan Wang , Lihao Wu , Yi Wang , Jun Dong . A distinction of gliomas at cellular and tissue level by surface-enhanced Raman scattering spectroscopy. Chinese Chemical Letters, 2024, 35(5): 109383-. doi: 10.1016/j.cclet.2023.109383
16. [16]
  Huihui LIU , Baichuan ZHAO , Chuanhui WANG , Zhi WANG , Congyun ZHANG . Green synthesis of MIL-101/Au composite particles and their sensitivity to Raman detection of thiram. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 2021-2030. doi: 10.11862/CJIC.20240059
17. [17]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
18. [18]
  Weiyu Chen , Zenghui Li , Chenguang Zhao , Lisha Zha , Junfeng Shi , Dan Yuan . Enzyme-modulate conformational changes in amphiphile peptide for selectively cell delivery. Chinese Chemical Letters, 2024, 35(12): 109628-. doi: 10.1016/j.cclet.2024.109628
19. [19]
  Jing-Jing Zhang , Lujun Lou , Rui Lv , Jiahui Chen , Yinlong Li , Guangwei Wu , Lingchao Cai , Steven H. Liang , Zhen Chen . Recent advances in photochemistry for positron emission tomography imaging. Chinese Chemical Letters, 2024, 35(8): 109342-. doi: 10.1016/j.cclet.2023.109342
20. [20]
  Shihong Wu , Ronghui Zhou , Hang Zhao , Peng Wu . Sonoafterglow luminescence for in vivo deep-tissue imaging. Chinese Chemical Letters, 2024, 35(10): 110026-. doi: 10.1016/j.cclet.2024.110026

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(997)
HTML views(91)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142