Citation: Yang Qianyu, Wang Maolin, Sun Yongbing, Peng Shengming, Ding Yanhuai, Yi Cao. Pre-incubated with BSA-complexed free fatty acids alters ER stress/autophagic gene expression by carboxylated multi-walled carbon nanotube exposure in THP-1 macrophages[J]. Chinese Chemical Letters, ;2019, 30(6): 1224-1228. doi: 10.1016/j.cclet.2019.03.042 shu

Pre-incubated with BSA-complexed free fatty acids alters ER stress/autophagic gene expression by carboxylated multi-walled carbon nanotube exposure in THP-1 macrophages

Yang Qianyu ^a,1 ,
Wang Maolin ^a,1 ,
Sun Yongbing ^b ,
Peng Shengming ^a ,
Ding Yanhuai ^a,*, ,
Yi Cao ^a,*,

a.
Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, China
b.
National Engineering Research Center for Solid Preparation Technology of Chinese Medicines, Jiangxi University of Traditional Chinese Medicines, Nanchang 330006, China

E-mail addresses:

yhding@xtu.edu.cn

caoyi39@xtu.edu.cn

caoyi39@126.com

Received Date: 21 February 2019
Revised Date: 22 March 2019
Accepted Date: 25 March 2019
Available Online: 27 June 2019

Figures(4)

Recently we found that multi-walled carbon nanotube (MWCNT) exposure alters the mRNA levels of endoplasmic reticulum (ER) stress/autophagic genes, but the impact of biological molecules on this response is unclear. Herein, we compared the different actions of carboxylated MWCNTs (c-MWCNTs) pre-incubated with bovine serum albumin (BSA) or BSA-complexed free fatty acid (denoted as FFA) on macrophages derived from THP-1 monocytes (denoted as THP-1 macrophages). C-MWCNTs exhibited increased diameter and hydrodynamic size as well as decreased absolute zeta potential value after preincubation with BSA or FFA, which suggested a coating effect. Cytotoxicity or oxidative stress were not significantly induced after exposure to BSA-or FFA-coated c-MWCNTs. BSA-pre-incubated c-MWCNTs significantly enhanced the expression of the ER stress gene, DDIT3 and the autophagic genes, ATG5, BECN1, and PLIN2, but the mRNA levels of these genes was significantly decreased by FFA-pre-incubated c-MWCNTs. FFA-pre-incubated c-MWCNTs induced significantly higher lipid accumulation and interleukin-6 (IL-6) protein level compared with BSA-pre-incubated c-MWCNTs, which suggested that FFA-pre-incubated c-MWCNTs may more effectively induce the formation of macrophage foam cells. Collectively, our data indicated that pre-incubation with FFA may influence c-MWCNT-induced ER stress/autophagic gene expression and foam cell formation in THP-1 macrophages.
- Multi-walled carbon nanotubes (MWCNTs),
- THP-1 macrophages,
- Endoplasmic reticulum (ER) stress,
- Autophagy,
- Foam cells
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