Citation: Zhou Xiaoman, Yang Shang, Yang Ganglong, Tan Zengqi, Guan Feng. Two-step derivatization and mass spectral distinction of α2, 3 and α2, 6 sialic acid linkages on N-glycans by MALDI-TOF[J]. Chinese Chemical Letters, ;2019, 30(3): 676-680. doi: 10.1016/j.cclet.2018.12.016 shu

Two-step derivatization and mass spectral distinction of α2, 3 and α2, 6 sialic acid linkages on N-glycans by MALDI-TOF

Zhou Xiaoman ^a ,
Yang Shang ^b ,
Yang Ganglong ^a ,
Tan Zengqi ^b ,
Guan Feng ^a,b,*,

a.
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
b.
Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, China

guanfeng@nwu.edu.cn

Received Date: 11 September 2018
Revised Date: 12 December 2018
Accepted Date: 3 December 2018
Available Online: 14 March 2018

Figures(4)

Sialylation is one of important glycosylation in human beings and plays an important role in cancer development. α2, 3-Linked and α2, 6-linked sialic acids are normally observed on the end of N-glycans and have different functions. Derivatization on sialic acid was designed to detect the different linkages by MALDI-TOF MS. In this study, a two-step derivatization by dimethylamine and ammonium hydroxide was improved to modify the sialic acid and made it easier to detect the different linkages of sialic acids on MALDI-TOF MS. Using this derivatization method, specific sialic acids linkages on N-glycans of protein samples such as fetuin and lactoferrinwere detected. For complex cell samples, increased α2, 3-linked and α2, 6-linked sialic acids on bi-antennary and tri-antennary N-glycans were observed in A549 cells induced by hypoxia environment. Taken together, our two-step derivatization of sialic acids offers a simple and accurate way to detect specific linkages on N-glycans with MALDI-TOF mass spectrometer.
- Sialic acid,
- N-Glycans,
- Derivatization,
- MALDI-TOF,
- Hypoxia
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