Citation: Huang Li, Zhang Di, Jiao Lirong, Su Enben, He Nongyue. A new quality control method for lateral flow assay[J]. Chinese Chemical Letters, ;2018, 29(12): 1853-1856. doi: 10.1016/j.cclet.2018.11.028 shu

A new quality control method for lateral flow assay

Huang Li ^a,b,c ,
Zhang Di ^a,b ,
Jiao Lirong ^a,b,c ,
Su Enben ^c ,
He Nongyue ^a,b,*,

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China
c.
Getein Biotechnology Co., Ltd., Nanjing 210000, China

nyhe@seu.edu.cn

Received Date: 20 November 2018
Revised Date: 27 November 2018
Accepted Date: 28 November 2018
Available Online: 28 December 2018

Figures(4)

Precision and repeatability are challenging issues in point of care testing (POCT) analysis. Herein, we proposed a lateral flow assay (LFA) based on internal quality control microspheres to realize the accurate diagnosis of HbA1c in human body. Fluorescein cy5 decorated microspheres are used as labels for HbA1c detection, and BSA-fluorescein isothiocyanate (FITC) decorated microspheres are used as internal quality control labels. One test line was employed in the strip for the detection of glycosylated hemoglobin (HbA1c). This method can eliminate the interference of environmental factors (temperature, humidity, etc.) to LFA in the process of chromatography, and improve the precision and accuracy of HbA1c detection. The CV for detection of low concentration HbA1c was 1.05%, and the CV for detection of high concentration HbA1c was 0.69%. We envision the method to have great prospect in in vitro diagnosis (IVD).
- Point of care testing,
- Lateral flow assay,
- HbA1c,
- Fluorescein isothiocyanate,
- Precision
1. [1]
  R.H. Tang, H. Yang, J.R. Choi, et al., Crit. Rev. Biotechnol. 37 (2016) 411-428.
2. [2]
  C. Parolo, A. Merkoci, Chem. Soc. Rev. 42 (2013) 450-457. doi: 10.1039/C2CS35255A
3. [3]
  W.C. Huang, K.H. Wu, H.C. Hung, et al., J. Nanosci. Nanotechnol. 18 (2018) 7190. doi: 10.1166/jnn.2018.16020
4. [4]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Sensor. Actuat. B -Chem. 252 (2017) 44-54. doi: 10.1016/j.snb.2017.05.148
5. [5]
  A. Murali, N. Haridharan, P.S. Babu, S. Jayaveni, S.N. Jaisankar, J. Nanosci. Nanotechnol. 18 (2018) 959-966. doi: 10.1166/jnn.2018.13959
6. [6]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Biosens. Bioelectron. 92 (2017) 87-94. doi: 10.1016/j.bios.2017.02.002
7. [7]
  Y. Jin, Q. Xue, D. He, X. Liu, S. Lu, J. Nanosci. Nanotechnol. 17 (2017) 6216-6223. doi: 10.1166/jnn.2017.14434
8. [8]
  H.S. Han, M.S. Ahmed, H. Jeong, S. Jeon, J. Electrochem. Soc. 162 (2015) B75-B80. doi: 10.1149/2.0651504jes
9. [9]
  W. Wang, L. Liu, L. Xu, et al., Part. Part. Syst. Charact. 33 (2016) 388-395. doi: 10.1002/ppsc.201500219
10. [10]
  Y. Huang, J. Tan, L.J. Cui, et al., Biosens. Bioelectron. 102 (2018) 560-567. doi: 10.1016/j.bios.2017.11.037
11. [11]
  R. Li, X. Zhang, C. Yang, et al., J. Nanosci. Nanotechnol. 18 (2018) 3800-3805. doi: 10.1166/jnn.2018.15257
12. [12]
  F. Chen, X. Zhang, X. Hu, P. Liu, H. Zhang, Nanosci. Nanotechnol. Lett. 10 (2018) 259-266. doi: 10.1166/nnl.2018.2621
13. [13]
  J. Yang, F. Nian, Z. Guo, T. Bai, G. Peng, Nanosci. Nanotechnol. Lett. 9 (2017) 1491-1496. doi: 10.1166/nnl.2017.2503
14. [14]
  Y. Xu, Y. Liu, Y. Wu, et al., Anal. Chem. 86 (2014) 5611-5614. doi: 10.1021/ac5010458
15. [15]
  S. Takalkar, K. Baryeh, G. Liu, Biosens. Bioelectron. 98 (2017) 147-154. doi: 10.1016/j.bios.2017.06.045
16. [16]
  L.L. Cao, Y. Jiang, Z. Chen, J. Nanosci. Nanotechnol. 18 (2018) 3067-3076. doi: 10.1166/jnn.2018.14674
17. [17]
  F. Zhao, L. Cao, Y. Liang, et al., J. Biomed. Nanotechnol. 13 (2017) 1300-1308. doi: 10.1166/jbn.2017.2415
18. [18]
  Y. Xing, X.Z. Feng, L.P. Zhang, et al., Int. J. Nanomed. Nanosurg. 12 (2017) 3171-3179. doi: 10.2147/IJN
19. [19]
  Y. Wu, W. Peng, Q. Zhao, et al., Chin. Chem. Lett. 28 (2017) 1881-1884. doi: 10.1016/j.cclet.2017.07.026
20. [20]
  M. Zarei, Biosens. Bioelectron. 98 (2017) 494-506. doi: 10.1016/j.bios.2017.07.024
21. [21]
  X. Gong, J. Cai, B. Zhang, et al., J. Mater. Chem. B -Mater. Biol. Med. 5 (2017) 5079-5091. doi: 10.1039/C7TB01049D
22. [22]
  M. Liu, K. Wu, L. Zhao, Y. Zhang, J. Biomed. Nanotechnol. 13 (2017) 381-392. doi: 10.1166/jbn.2017.2354
23. [23]
  J. Fan, X. Yu, L. He, et al., Nanosci. Nanotechnol. Lett. 9 (2017) 1478-1486. doi: 10.1166/nnl.2017.2501
24. [24]
  H. Li, X.M. Xie, Chin. Chem. Lett. 29 (2018) 161-165. doi: 10.1016/j.cclet.2017.06.001
25. [25]
  Standards of the Pharmaceutical Industry of the People's Republic of China, China Standards Press, 2018(YY/T 1605-2018).
26. [26]
  D. Zhang, L. Huang, B. Liu, et al., Biosens. Bioelectron. 106 (2018) 204-211. doi: 10.1016/j.bios.2018.01.062
27. [27]
  D. Zhang, L. Huang, B. Liu, et al., Sensor. Actuat. B -Chem. 277 (2018) 502-509. doi: 10.1016/j.snb.2018.09.044
28. [28]
  Y. Du, L. Wang, W. Wang, et al., J. Biomed. Nanotechnol. 14 (1) (2018) 161-167.
29. [29]
  L. Li, G. Rao, X. Lv, et al., J. Biomed. Opt. 23 (2018) 1-4.
30. [30]
  S. Ma, L. Li, M. She, et al., Chin. Chem. Lett. 28 (2017) 2014-2018. doi: 10.1016/j.cclet.2017.09.027
31. [31]
  S. Song, N. Liu, Z. Zhao, et al., Anal. Chem. 86 (2014) 4995-5001. doi: 10.1021/ac500540z
1. [1]
  Bingyan Wen , Wenjing Zhang , Qi-Wei Zhang , Yang Tian . Cyclodextrin-mediated efficient antibody immobilization on upconversion nanoparticles for CKD biomarker sensing via luminescent lateral flow assay. Chinese Chemical Letters, 2026, 37(3): 111459-. doi: 10.1016/j.cclet.2025.111459
2. [2]
  Zhaorui Song , Qiulian Hao , Bing Li , Yuwei Yuan , Shanshan Zhang , Yongkuan Suo , Hai-Hao Han , Zhen Cheng . NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers. Chinese Chemical Letters, 2025, 36(1): 109834-. doi: 10.1016/j.cclet.2024.109834
3. [3]
  Chen Liu , Tianqi Zhao , Jialing Zhou , Xiaoyun Hu , Dinghao Pan , Jinlong Li , Wei Li , Zhihui Dai . Optical lateral flow immune assay technology for body fluid sensing. Chinese Chemical Letters, 2026, 37(1): 110967-. doi: 10.1016/j.cclet.2025.110967
4. [4]
  Shaoqiang Wen , Lang Zhang , Zhan Huang , Meng Chen , Yuzhi Xu , Xing Huang , Zhou Zhang , Xiaoyong Zou , Zong Dai , Si-Yang Liu . Aptamer&MOF-functionalized multichannel paper chip for point-of-care testing of small extracellular vesicle membrane protein profile. Chinese Chemical Letters, 2026, 37(2): 111333-. doi: 10.1016/j.cclet.2025.111333
5. [5]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
6. [6]
  Panpan Sun , Qian Li , Ningshuang Gao , Mingyue Luo , Wenzhuo Chang , Baodui Wang , Xiaoquan Lu , Zhonghua Xue . Solid state luminescent-enabled lateral flow immunoassay with highly fluorescence performance for rapid and quantitative detection of C-reactive protein. Chinese Chemical Letters, 2025, 36(10): 110801-. doi: 10.1016/j.cclet.2024.110801
7. [7]
  Haolin Zhang , Kai Feng , Guisen Li , Weijiao Chen , Yuan Shao , Jiayu Ding , Mingming Zheng , Kai Yuan , Xiaolian Sun , Peng Yang . Carbonic anhydrase IX-targeted SMDCs for cancer precision treatment. Chinese Chemical Letters, 2026, 37(4): 111047-. doi: 10.1016/j.cclet.2025.111047
8. [8]
  Yuxin Xiao , Xiaowei Wang , Yutong Yin , Fangchao Yin , Jinchao Li , Zhiyuan Hou , Mashooq Khan , Rusong Zhao , Wenli Wu , Qiongzheng Hu . Distance-based lateral flow biosensor for the quantitative detection of bacterial endotoxin. Chinese Chemical Letters, 2024, 35(12): 109718-. doi: 10.1016/j.cclet.2024.109718
9. [9]
  Haijiang Gong , Qingtan Zeng , Shili Gai , Yaqian Du , Jing Zhang , Qingyu Wang , He Ding , Lichun Wu , Anees Ahmad Ansari , Piaoping Yang . Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay. Chinese Chemical Letters, 2025, 36(5): 110059-. doi: 10.1016/j.cclet.2024.110059
10. [10]
  Junfei Yang , Ke Wang , Shuxin Sun , Tianqi Pei , Junxiu Li , Xunwei Gong , Cuixia Zheng , Yun Zhang , Qingling Song , Lei Wang . A "spore-like" oral nanodrug delivery platform for precision targeted therapy of inflammatory bowel disease. Chinese Chemical Letters, 2025, 36(3): 110180-. doi: 10.1016/j.cclet.2024.110180
11. [11]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
12. [12]
  Xiaoliu Liang , Chunliu Huang , Hui Liu , Hu Chen , Jiabao Shou , Hongwei Cheng , Gang Liu . Natural hydrogel dressings in wound care: Design, advances, and perspectives. Chinese Chemical Letters, 2024, 35(10): 109442-. doi: 10.1016/j.cclet.2023.109442
13. [13]
  Benjian Xin , Rui Wang , Lili Liu , Zhiqiang Niu . Metal-organic framework derived MnO@C/CNTs composite for high-rate lithium-based semi-solid flow batteries. Chinese Journal of Structural Chemistry, 2023, 42(11): 100116-100116. doi: 10.1016/j.cjsc.2023.100116
14. [14]
  Hui Jin , Qin Cai , Peiwen Liu , Yan Chen , Derong Wang , Weiping Zhu , Yufang Xu , Xuhong Qian . Multistep continuous flow synthesis of Erlotinib. Chinese Chemical Letters, 2024, 35(4): 108721-. doi: 10.1016/j.cclet.2023.108721
15. [15]
  Peiwen Liu , Fang Zhao , Jing Zhang , Yunpeng Bai , Jinxing Ye , Bo Bao , Xinggui Zhou , Li Zhang , Changlu Zhou , Xinhai Yu , Peng Zuo , Jianye Xia , Lian Cen , Yangyang Yang , Guoyue Shi , Lin Xu , Weiping Zhu , Yufang Xu , Xuhong Qian . Micro/nano flow chemistry by Beyond Limits Manufacturing. Chinese Chemical Letters, 2024, 35(5): 109020-. doi: 10.1016/j.cclet.2023.109020
16. [16]
  Huan Yao , Jian Qin , Yan-Fang Wang , Song-Meng Wang , Liu-Huan Yi , Shi-Yao Li , Fangfang Du , Liu-Pan Yang , Li-Li Wang . Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay. Chinese Chemical Letters, 2024, 35(6): 109154-. doi: 10.1016/j.cclet.2023.109154
17. [17]
  Chunyu Yan , Qinglong Qiao , Wei Zhou , Xuelian Zhou , Yonghui Chen , Lu Miao , Zhaochao Xu . FRET-based in vitro assay for rapid detecting of SARS-CoV-2 entry inhibitors. Chinese Chemical Letters, 2025, 36(5): 110258-. doi: 10.1016/j.cclet.2024.110258
18. [18]
  Jiayu Huang , Kuan Chang , Qi Liu , Yameng Xie , Zhijia Song , Zhiping Zheng , Qin Kuang . Fe-N-C nanostick derived from 1D Fe-ZIFs for Electrocatalytic oxygen reduction. Chinese Journal of Structural Chemistry, 2023, 42(10): 100097-100097. doi: 10.1016/j.cjsc.2023.100097
19. [19]
  Qin Cheng , Ming Huang , Qingqing Ye , Bangwei Deng , Fan Dong . Indium-based electrocatalysts for CO₂ reduction to C1 products. Chinese Chemical Letters, 2024, 35(6): 109112-. doi: 10.1016/j.cclet.2023.109112
20. [20]
  Huashan Huang , Jingze Chen , Luyun Zhang , Hong Yan , Siqi Li , Fen-Er Chen . Oscillatory flow reactor facilitates fast photochemical Wolff rearrangement toward synthesis of α-substituted amides in flow. Chinese Chemical Letters, 2025, 36(2): 109992-. doi: 10.1016/j.cclet.2024.109992

Get Citation

PDF

XML

Metrics

PDF Downloads(21)
Abstract views(1962)
HTML views(150)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142