Citation: Zhang Mengge, Wen Ming, Xiong Ying, Zhang Longhua, Tian Changlin. In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺[J]. Chinese Chemical Letters, ;2018, 29(10): 1509-1512. doi: 10.1016/j.cclet.2018.04.026 shu

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺

Zhang Mengge ^a ,
Wen Ming ^a ,
Xiong Ying ^a ,
Zhang Longhua ^a,, ,
Tian Changlin ^a,b,*,,

a.
National Laboratory of Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
b.
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China

Corresponding author: Zhang Longhua, zlhustc@ustc.edu.cn Tian Changlin, cltian@ustc.edu.cn

Received Date: 7 April 2018
Revised Date: 19 April 2018
Accepted Date: 23 April 2018
Available Online: 24 October 2018

Figures(4)

Fluorescence microscopy, as a sensitive method to detect microenvironment of molecules, is widely used in protein conformation and dynamic studies in live cells. Fluorescence lifetime imaging microscopy (FLIM), which is independent of fluorophore concentrations, scattering and bleaching, is a suitable tool to analyze membrane proteins in a single cell. Ferroportin (FPN), a multi-ion exporter in vertebrates, was modulated by metal ions with unknown mechanism. Herein, we fused green fluorescence protein on Cterminal of FPN (FPN-eGFP) and applied fluorescence lifetime to monitor conformation changes of FPN in a live cell. The fluorescence lifetime distribution showed a shift to shorter lifetime upon Mn²⁺ treatment, suggesting a preference conformation of FPN in Mn²⁺ exposure. It is also observed that the lifetime (rather than intensity) measurement was not strongly influenced by laser power. The observed fluorescence lifetime changes of FPN-eGFP upon Mn²⁺ treatments indicated that extracellular metal ions can modulate FPN through conformation exchanges between several different states.
- Fluorescence lifetime imaging microscopy,
- In cell analysis of conformation change,
- Ferroprotin
1. [1]
  B.A. Griffin, S.R. Adams, R.Y. Tsien, Science 281(1998) 269-272. doi: 10.1126/science.281.5374.269
2. [2]
  P.I. Bastiaens, A. Squire, Trends Cell Biol. 9(1999) 48-52. doi: 10.1016/S0962-8924(98)01410-X
3. [3]
  Q. Zhang, X. Dong, K.P. Wang, et al., Chin. Chem. Lett. 28(2017) 777-781. doi: 10.1016/j.cclet.2017.03.001
4. [4]
  S. Liu, P. Lv, D. Li, et al., Chem. Commun. (Camb.) 51(2015) 15971-15974. doi: 10.1039/C5CC06124E
5. [5]
  P. Ning, W.J. Wang, M. Chen, Y. Feng, X.M. Meng, Chin. Chem. Lett. 28(2017) 1943-1951. doi: 10.1016/j.cclet.2017.09.026
6. [6]
  J.M. Beechem, L. Brand, Annu. Rev. Biochem. 54(1985) 43-71. doi: 10.1146/annurev.bi.54.070185.000355
7. [7]
  T. Ha, A.Y. Ting, J. Liang, et al., Proc. Natl. Acad. Sci. U. S. A. 96(1999) 893-898. doi: 10.1073/pnas.96.3.893
8. [8]
  T.W.J. Gadella, T.M. Jovin, R.M. Clegg, Biophys. Chem. 48(1993) 221-239. doi: 10.1016/0301-4622(93)85012-7
9. [9]
  A. Donovan, A. Brownlie, Y. Zhou, et al., Nature 403(2000) 776-781. doi: 10.1038/35001596
10. [10]
  G. Hetet, I. Devaux, N. Soufir, B. Grandchamp, C. Beaumont, Blood 102(2003) 1904-1910. doi: 10.1182/blood-2003-02-0439
11. [11]
  A. Donovan, C.A. Lima, J.L. Pinkus, et al., Cell. Metab. 1(2005) 191-200. doi: 10.1016/j.cmet.2005.01.003
12. [12]
  H. Zoller, R.O. Koch, I. Theurl, et al., Gastroenterology 120(2001) 1412-1419. doi: 10.1053/gast.2001.24033
13. [13]
  E. Nemeth, M.S. Tuttle, J. Powelson, et al., Science 306(2004) 2090-2093. doi: 10.1126/science.1104742
14. [14]
  C. Delaby, N. Pilard, A.S. Goncalves, C. Beaumont, F. Canonne-Hergaux, Blood 106(2005) 3979-3984. doi: 10.1182/blood-2005-06-2398
15. [15]
  D.L. Zhang, T. Senecal, M.C. Ghosh, et al., Blood 118(2011) 2868-2877. doi: 10.1182/blood-2011-01-330241
16. [16]
  M.S. Madejczyk, N. Ballatori, Biochim. Biophys. Acta 1818(2012) 651-657. doi: 10.1016/j.bbamem.2011.12.002
17. [17]
  C.J. Mitchell, A. Shawki, T. Ganz, E. Nemeth, B. Mackenzie, Am. J. Physiol. Cell Physiol. 306(2014) C450-459. doi: 10.1152/ajpcell.00348.2013
18. [18]
  K.M. Erikson, M. Aschner, Neurochem. Int. 43(2003) 475-480. doi: 10.1016/S0197-0186(03)00037-8
19. [19]
  H. Gunshin, B. Mackenzie, U.V. Berger, et al., Nature 388(1997) 482-488. doi: 10.1038/41343
20. [20]
  E.A. Malecki, B.M. Cook, A.G. Devenyi, J.L. Beard, J.R. Connor, J. Neurol. Sci. 170(1999) 112-118. doi: 10.1016/S0022-510X(99)00203-8
21. [21]
  Y.A. Seo, M. Wessling-Resnick, FASEB J. 29(2015) 2726-2733. doi: 10.1096/fj.14-262592
22. [22]
  Z. Yin, H. Jiang, E.S. Lee, et al., J. Neurochem. 112(2010) 1190-1198. doi: 10.1111/jnc.2010.112.issue-5
23. [23]
  E.A. Smith, P. Newland, K.G. Bestwick, N. Ahmed, Elem. Med. Biol. 27(2013) 65-69. doi: 10.1016/j.jtemb.2012.07.002
24. [24]
  R. Taniguchi, H.E. Kato, J. Font, et al., Nat. Commun. 6(2015) 8545. doi: 10.1038/ncomms9545
25. [25]
  A. Periasamy, M. Elangovan, E. Elliott, D.L. Brautigan, Methods Mol. Biol. 183(2002) 89-100.
26. [26]
  H.C. Gerritsen, M.A. Asselbergs, A.V. Agronskaia, W.G. van Sark, J. Microsc. 206(2002) 218-224. doi: 10.1046/j.1365-2818.2002.01031.x
1. [1]
  Can Wang , Zhe Sun , Donghan Ma . Review of imaging buffers used in stochastic optical reconstruction microscopy. Chinese Chemical Letters, 2025, 36(9): 110677-. doi: 10.1016/j.cclet.2024.110677
2. [2]
  Zeyin Chen , Jiaju Shi , Yusheng Zhou , Peng Zhang , Guodong Liang . Polymer microparticles with ultralong room-temperature phosphorescence for visual and quantitative detection of oxygen through phosphorescence image and lifetime analysis. Chinese Chemical Letters, 2025, 36(5): 110629-. doi: 10.1016/j.cclet.2024.110629
3. [3]
  Luolin Wang , Xing Liang , Hanwen Chi , Weiying Lin . A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice. Chinese Chemical Letters, 2025, 36(12): 110962-. doi: 10.1016/j.cclet.2025.110962
4. [4]
  Zixuan Chen , Yafeng Wu , Zhaoyan Tian , Zhaohan Wang , Weiwei Liu , Songqin Liu . A reproducible hybrid membrane for in situ analysis of cell secretions with a wide size range. Chinese Chemical Letters, 2025, 36(12): 110917-. doi: 10.1016/j.cclet.2025.110917
5. [5]
  Yuxin Li , Chengbin Liu , Qiuju Li , Shun Mao . Fluorescence analysis of antibiotics and antibiotic-resistance genes in the environment: A mini review. Chinese Chemical Letters, 2024, 35(10): 109541-. doi: 10.1016/j.cclet.2024.109541
6. [6]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
7. [7]
  Kaimin WANG , Xiong GU , Na DENG , Hongmei YU , Yanqin YE , Yulu MA . Synthesis, structure, fluorescence properties, and Hirshfeld surface analysis of three Zn(Ⅱ)/Cu(Ⅱ) complexes based on 5-(dimethylamino) isophthalic acid. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1397-1408. doi: 10.11862/CJIC.20240009
8. [8]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
9. [9]
  Zhixue Liu , Haiqi Chen , Lijuan Guo , Xinyao Sun , Zhi-Yuan Zhang , Junyi Chen , Ming Dong , Chunju Li . Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging. Chinese Chemical Letters, 2024, 35(9): 109666-. doi: 10.1016/j.cclet.2024.109666
10. [10]
  Qian Ren , Xue Dai , Ran Cen , Yang Luo , Mingyang Li , Ziyun Zhang , Qinghong Bai , Zhu Tao , Xin Xiao . A cucurbit[8]uril-based supramolecular phosphorescent assembly: Cell imaging and sensing of amino acids in aqueous solution. Chinese Chemical Letters, 2024, 35(12): 110022-. doi: 10.1016/j.cclet.2024.110022
11. [11]
  Zhe Li , Haozhi Lei , Zhiqiang Ren , Cheng Wang , Qian Xia , Weihong Tan . Enhancing the stability of ⁶⁸Ga-labeled RNA aptamers for pancreatic β-cell and insulinoma imaging through nucleoside modifications. Chinese Chemical Letters, 2025, 36(10): 110804-. doi: 10.1016/j.cclet.2024.110804
12. [12]
  Yiling Li , Zekun Gao , Xiuxiu Yue , Minhuan Lan , Xiuli Zheng , Benhua Wang , Shuang Zhao , Xiangzhi Song . FRET-based two-photon benzo[a] phenothiazinium photosensitizer for fluorescence imaging-guided photodynamic therapy. Chinese Chemical Letters, 2024, 35(7): 109133-. doi: 10.1016/j.cclet.2023.109133
13. [13]
  Peide Zhu , Yangjia Liu , Yaoyao Tang , Siqi Zhu , Xinyang Liu , Lei Yin , Quan Liu , Zhiqiang Yu , Quan Xu , Dixian Luo , Juncheng Wang . Bi-doped carbon quantum dots functionalized liposomes with fluorescence visualization imaging for tumor diagnosis and treatment. Chinese Chemical Letters, 2024, 35(4): 108689-. doi: 10.1016/j.cclet.2023.108689
14. [14]
  Kangmin Wang , Liqiu Wan , Jingyu Wang , Chunlin Zhou , Ke Yang , Liang Zhou , Bijin Li . Multifunctional 2-(2′-hydroxyphenyl)benzoxazoles: Ready synthesis, mechanochromism, fluorescence imaging, and OLEDs. Chinese Chemical Letters, 2024, 35(10): 109554-. doi: 10.1016/j.cclet.2024.109554
15. [15]
  Biao Huang , Tao Tang , Fushou Liu , Shi-Hui Chen , Zhi-Ling Zhang , Mingxi Zhang , Ran Cui . Quantum dots boost large-view NIR-Ⅱ imaging with high fidelity for fluorescence-guided tumor surgery. Chinese Chemical Letters, 2024, 35(12): 109694-. doi: 10.1016/j.cclet.2024.109694
16. [16]
  Du Liu , Yuyan Li , Hankun Zhang , Benhua Wang , Chaoyi Yao , Minhuan Lan , Zhanhong Yang , Xiangzhi Song . Three-in-one erlotinib-modified NIR photosensitizer for fluorescence imaging and synergistic chemo-photodynamic therapy. Chinese Chemical Letters, 2025, 36(2): 109910-. doi: 10.1016/j.cclet.2024.109910
17. [17]
  Zhoupeng Zheng , Shengyi Gong , Qianhua Li , Shiya Zhang , Guoqiang Feng . Lipid droplets and gallbladder targeted fluorescence probe for ratiometric NO imaging in gallstones disease models. Chinese Chemical Letters, 2025, 36(5): 110191-. doi: 10.1016/j.cclet.2024.110191
18. [18]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
19. [19]
  Jianqiu Li , Yi Zhang , Songen Liu , Jie Niu , Rong Zhang , Yong Chen , Yu Liu . Cucurbit[8]uril-based non-covalent heterodimer realized NIR cell imaging through topological transformation from nanowire to nanorod. Chinese Chemical Letters, 2024, 35(10): 109645-. doi: 10.1016/j.cclet.2024.109645
20. [20]
  Wanxin Li , Wenxing Gao , Mengyao Wen , Zecheng He , Li Shang . Controllable synthesis of selenolate ligand-costabilized water-soluble near-infrared fluorescent gold nanoclusters for cell imaging. Chinese Chemical Letters, 2025, 36(10): 110803-. doi: 10.1016/j.cclet.2024.110803

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(1415)
HTML views(76)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+

Metrics

通讯作者: 陈斌, bchen63@163.com

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺