Citation: Zhou Bin, Faridoon, Tian Xiaobo, Li Jian, Guan Dongliang, Zheng Xing, Guo Yu, Huang Wei. On-resin peptide ligation via C-terminus benzyl ester[J]. Chinese Chemical Letters, ;2018, 29(7): 1123-1126. doi: 10.1016/j.cclet.2018.03.021 shu

On-resin peptide ligation via C-terminus benzyl ester

Zhou Bin ^a,b ,
Faridoon ^b,c,**,, ,
Tian Xiaobo ^b ,
Li Jian ^b,c ,
Guan Dongliang ^b,c ,
Zheng Xing ^a ,
Guo Yu ^a,, ,
Huang Wei ^b,c,**,,

a.
Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China
b.
CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Center for Biotherapeutics Discovery Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
c.
University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: Faridoon, faridoon619@yahoo.com Guo Yu, guoyuhy@126.com Huang Wei, huangwei@simm.ac.cn

^**

Received Date: 8 February 2018
Revised Date: 7 March 2018
Accepted Date: 16 March 2018
Available Online: 27 July 2018

Figures(5)

Here, we report a new approach of on-resin peptide ligation using C-terminal benzyl ester as the stabilized precursor of thioester, which enables both N-terminal elongation and C-terminal peptide ligation on a Rink Amide resin. On-resin native chemical ligation and auxiliary-assisted peptide ligation were successfully achieved. This method is compatible to both protected and unprotected peptide fragments and has potential application in poor water-soluble peptide ligation.
- On-resin peptide ligation,
- Peptide benzyl ester,
- Native chemical ligation,
- Auxiliary-assisted peptide ligation,
- Peptide elongation
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