Citation: Duan Houna, Ding Yu, Huang Chusen, Zhu Weiping, Wang Rui, Xu Yufang. A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells[J]. Chinese Chemical Letters, ;2019, 30(1): 55-57. doi: 10.1016/j.cclet.2018.03.016 shu

A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

Duan Houna ^a ,
Ding Yu ^a ,
Huang Chusen ^b,, ,
Zhu Weiping ^a,, ,
Wang Rui ^c ,
Xu Yufang ^a

a.
State Key Laboratory of Bioreactor Engineerring, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
b.
The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
c.
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: Huang Chusen, huangcs@shnu.edu.cn Zhu Weiping, wpzhu@ecust.edu.cn
Received Date: 27 January 2018
Revised Date: 9 March 2018
Accepted Date: 12 March 2018
Available Online: 13 January 2018

Figures(4)

Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn²⁺ binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn²⁺ with the association constant of 4.9×10⁸ L/mol, and the detection limit of 15 nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H₂O₂ stimulation in SH-SY5Y cells, endogenous release of Zn²⁺ was observed.
- Naphthalimide,
- Zinc ions,
- Cellular imaging,
- Lysosome,
- Fluorescent probe
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