Citation: Zhu Paijin, Zhang Yiying, Xu Shuxia, Zhang Xinfeng. G-quadruplex-assisted enzyme strand recycling for amplified label-free fluorescent detection of UO₂²⁺[J]. Chinese Chemical Letters, ;2019, 30(1): 58-62. doi: 10.1016/j.cclet.2018.02.003 shu

G-quadruplex-assisted enzyme strand recycling for amplified label-free fluorescent detection of UO₂²⁺

a.
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China
b.
College of Environment and Ecology, Chengdu University of Technology, Chengdu 610059, China

Corresponding author: Xu Shuxia, xushux@cdut.edu.cn Zhang Xinfeng, zhangxinfeng09@cdut.cn
Received Date: 25 December 2017
Revised Date: 31 January 2018
Accepted Date: 3 February 2018
Available Online: 8 January 2018

Figures(5)

DNAzyme that can catalytically cleave of substrate DNA has shown to be attractive for amplified detection in biosensing events. During the catalytic process, the recycling of enzyme strand of DNAzyme is critically important. In this work, a G-quadruplex-assisted enzyme strand recycling strategy was developed for amplified label-free fluorescent detection of uranyl ion (UO₂²⁺). The DNAzyme was activated by the target UO₂²⁺ and further cleaved the substrate strand that contained the G-quadruplex sequence. The following formation of G-quadruplex helps the separation between the enzyme strand and the cleaved substrate strand, thus improving the recycle use of the enzyme strand. Such strategy allowed lablel-free detection of 0.2-200 ng/mL UO₂²⁺ via SYBR green Ⅰ (SG)-based fluorescence. The detection limit (3δ) is as low as 0.06 ng/mL (about 0.2 nmol/L), comparable to those obtained by ICP-MS and labeled DNAzyme. It was applied for detection of UO₂²⁺ in spiked environmental water samples with recoveries in the range of 96%-103%. This biosensor, with the advantages of simplicity and high sensitivity, is an appealing tool for fast detection of UO₂²⁺ in environmental water samples.
- Uranyl ion,
- DNAzyme,
- SYBR green Ⅰ,
- Fluorescence
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

G-quadruplex-assisted enzyme strand recycling for amplified label-free fluorescent detection of UO22+

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通讯作者: 陈斌, bchen63@163.com

G-quadruplex-assisted enzyme strand recycling for amplified label-free fluorescent detection of UO₂²⁺