Citation: Wu Zengnan, Xu Ning, Li Weiwei, Lin Jin-Ming. A membrane separation technique for optimizing sample preparation of MALDI-TOF MS detection[J]. Chinese Chemical Letters, ;2019, 30(1): 95-98. doi: 10.1016/j.cclet.2018.01.048 shu

A membrane separation technique for optimizing sample preparation of MALDI-TOF MS detection

Wu Zengnan ^a,b ,
Xu Ning ^b ,
Li Weiwei ^b ,
Lin Jin-Ming ^b,,

a.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
b.
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China

Corresponding author: Lin Jin-Ming, jmlin@mail.tsinghua.edu.cn
Received Date: 25 November 2017
Revised Date: 20 January 2018
Accepted Date: 24 January 2018
Available Online: 31 January 2018

Figures(5)

The performance of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) is frequently compromised by the high limited of detection, heterogeneous distribution of matrix and analyte deposits and time-consuming drying time during the conventional drop drying procedure. Here, we describe a method based on membrane separation technology to assist in the drying of nanomaterials to rapidly make homogeneous sample spots and to enhance the mass signal. We demonstrate that the sample can be dried evenly in a few seconds and MS signal can be greatly optimized by using membrane separation technology. Enrichment with nanomaterials greatly enhances analyte signal strength while membrane separation method avoids the time-consuming requirements of searching for "sweet spots".
- MALDI-MS,
- Membrane separation,
- Nanomaterial,
- Mastoparan,
- Insulin,
- Cytochrome C
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