Citation: Peng Luxin, Xu Yongxian, Zou Peng. Genetically-encoded voltage indicators[J]. Chinese Chemical Letters, ;2017, 28(10): 1925-1928. doi: 10.1016/j.cclet.2017.09.037 shu

Genetically-encoded voltage indicators

Peng Luxin ^a,c,1 ,
Xu Yongxian ^b,c,1 ,
Zou Peng ^a,b,c,,

a.
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
b.
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
c.
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China

Corresponding author: Zou Peng, zoupeng@pku.edu.cn

Received Date: 11 July 2017
Revised Date: 14 September 2017
Accepted Date: 14 September 2017
Available Online: 18 October 2017

Figures(2)

A holy grail in neuroscience is to understand how brain functions arise from neural network-level electrical activities. Voltage imaging allows for the direct visualization of electrical signaling at high spatial and temporal resolutions across a large neuronal population. Central to this technique is a palette of genetically-encoded fluorescent probes with fast and sensitive voltage responses. In this review, we chronicle the development and applications of genetically-encoded voltage indicators (GEVIs) over the past two decades, with a primary focus on the structural design that harness the power of fluctuating transmembrane electric fields. We hope this article will inform chemical biologists and protein engineers of the GEVI history and inspire novel design ideas.
- Fluorescent probe,
- Protein engineering,
- Voltage imaging,
- Biosensor,
- FRET
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