Citation: Guo Limin, Zhang Ziqi, Tang Yanli. Cationic conjugated polymers as signal reporter for label-free assay based on targets-mediated aggregation of perylene diimide quencher[J]. Chinese Chemical Letters, ;2018, 29(2): 305-308. doi: 10.1016/j.cclet.2017.08.032 shu

Cationic conjugated polymers as signal reporter for label-free assay based on targets-mediated aggregation of perylene diimide quencher

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China

Corresponding author: Tang Yanli, yltang@snnu.edu.cn
Received Date: 16 June 2017
Revised Date: 10 August 2017
Accepted Date: 15 August 2017
Available Online: 23 February 2017

Figures(4)

Herein, we reported a new label-free and fluorescence turn-on biosensor based on cationic conjugated poly(9, 9-bis(6'-N, N, N-trimethylammonium)hexyl)fluorine phenylene) (PFP) and perylene diimide derivatives (PDI). Cationic PFP, single-stranded nucleic acid and PDI were used as signal reporter, probe and fluorescence quencher, respectively. In the presence of nucleic acids, they form complexes with PFP and PDI through strong electrostatic attraction interactions, resulting in PDI aggregating on nucleic acids and fluorescence of PFP being quenched. When nucleic acids are hydrolyzed by enzymes or their conformation is changed via recognizing targets, the effective aggregation of PDI is disrupted and the quenching ability is decreased. Thus the fluorescence of PFP recovers significantly. By taking advantage of the mechanism, we construct a new biosensor for endonuclease and small molecules detection. Here, S1 nuclease and bisphenol A are used as model systems. The detection limit of the S1 nuclease and BPA are 1.0×10^-6 U/mL and 0.05 ng/mL, respectively. Our method is sensitive, cost-effective and simple, and provides a new platform for bioanalysis.
- Conjugated polymers,
- Perylene diimide derivatives,
- Label-free,
- S1 nuclease,
- Bisphenol A,
- Fluorescence turn-on
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