Citation: He Yangyang, Li Zhanxian, Jia Qingyan, Shi Bingjie, Zhang Hongyan, Wei Liuhe, Yu Mingming. Ratiometric fluorescent detection of acidic pH in lysosome with carbon nanodots[J]. Chinese Chemical Letters, ;2017, 28(10): 1969-1974. doi: 10.1016/j.cclet.2017.07.027 shu

Ratiometric fluorescent detection of acidic pH in lysosome with carbon nanodots

a.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China
b.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China

Corresponding author: Li Zhanxian, lizx@zzu.edu.cn Zhang Hongyan, zhanghongyan@mail.ipc.ac.cn Yu Mingming, yumm@zzu.edu.cn
Received Date: 3 May 2017
Revised Date: 13 July 2017
Accepted Date: 27 July 2017
Available Online: 4 October 2017

Figures(10)

It is significant for cell physiology to keep the homeostasis of pH, and it is highly demanded to develop ratiometric fluorescent sensors toward pH. In this work, under mild condition, through the electrostatic interaction between carbon nanodots (CDs) and organic molecules, two novel ratiometric fluorescence hybrid nanosensors were fabricated for sensing acidic pH. These nanohybrid systems possess dual emission peaks at 455 and 527 nm under a single excitation wavelength of 380 nm in acidic pH condition. With the increasing of pH, the fluorescence of the 1, 8-naphthalimide derivative completely quenches, while the blue fluorescence of CDs keeps constant. Furthermore, the CDs organic molecular nanohybrids exhibit excellent anti-disturbance ability, reversible pH sensing ability, and a linear response range in wide pH range respectively. Besides the ability to target lysosome, with one of the nanosensor, stimulated pH change has been successfully tracked in a ratiometric manner via fluorescence imaging.
- Acidic pH,
- Fluorescence,
- Carbon dot,
- Hybrid nanosensor,
- Lysosome-targeted
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沈阳化工大学材料科学与工程学院沈阳 110142