Citation: Wu Yudong, Peng Weipan, Zhao Qian, Piao Jiafang, Zhang Bo, Wu Xiaoli, Wang Hanjie, Shi Zhihong, Gong Xiaoqun, Chang Jin. Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen[J]. Chinese Chemical Letters, ;2017, 28(9): 1881-1884. doi: 10.1016/j.cclet.2017.07.026 shu

Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen

Wu Yudong ^a,1 ,
Peng Weipan ^a,1 ,
Zhao Qian ^a ,
Piao Jiafang ^a ,
Zhang Bo ^a ,
Wu Xiaoli ^a ,
Wang Hanjie ^a ,
Shi Zhihong ^b ,
Gong Xiaoqun ^a,, ,
Chang Jin ^a,,

a.
School of Life Sciences, School of Materials Science and Engineering, Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology(Tianjin), Tianjin 300072, China
b.
Department of Neurology, and Tianjin Neurosurgery Institute, Tianjin Huanhu Hospital, Tianjin 300060, China

Corresponding author: Gong Xiaoqun, gongxiaoqun@tju.edu.cn Chang Jin, jinchang@tju.edu.cn

Received Date: 2 May 2017
Revised Date: 21 July 2017
Accepted Date: 26 July 2017
Available Online: 29 September 2017

Figures(4)

At present, many researchers focused on the point-of-care testing (POCT), a method of disease markers detection without large-scale instruments and specialized persons. However, most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection. Herein, we developed a newly QD-immune fluorescence test strips (QD-IFTS) based on quantum dots (QDs) as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen (CEA), a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis. And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS. Under the optimized reaction conditions, chromatographic time of the newly QD-IFTS was only 25 min, sample volume of the newly QD-IFTS was only 40 μL and the LOD of the newly QD-IFTS was 0.72 ng/mL. In addition, the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples, and the results revealed great potential in clinical POCT of other biomarkers.
- Point-of-care testing,
- Immunochromatography assay,
- Quantum dots,
- Carcinoembryonic antigen,
- Quantitative detection
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