Citation: Zhang Qian, Dong Xue, Wang Kun-Peng, Zhu Ting-Ting, Sun Feng-Nan, Meng Shu-Xian, Feng Ya-Qing. Glycine-conjugated porphyrin fluorescent probe with iRGD for live cell imaging[J]. Chinese Chemical Letters, ;2017, 28(4): 777-781. doi: 10.1016/j.cclet.2017.03.001 shu

Glycine-conjugated porphyrin fluorescent probe with iRGD for live cell imaging

Zhang Qian ^a,b ,
Dong Xue ^a,b ,
Wang Kun-Peng ^a,b ,
Zhu Ting-Ting ^a,b ,
Sun Feng-Nan ^a,b ,
Meng Shu-Xian ^a,b,, ,
Feng Ya-Qing ^a,b

a.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
b.
Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, China

Corresponding author: Meng Shu-Xian, msxmail@tju.edu.cn
Received Date: 3 January 2017
Revised Date: 13 January 2017
Accepted Date: 2 March 2017
Available Online: 4 April 2017

Figures(5)

A porphyrin modified by glycine has been synthesized and developed as a near-infrared(NIR) fluorescence probe to detect tumor.Porphyrins'longwavelength emission at ~650 nm can efficiently avoid the spectral crosstalk with Spontaneous fluorescence in the visible light region.A disulfide-based cyclic RGD peptide named iRGD c(CRGDKGPDC), a tumor homing peptide, harbors a cryptic C-end Rule (CendR)motif that is responsible for neuropilin-1(NRP-1) binding and for triggering extravasation and tumor penetration of the peptide to improve the imaging sensitivity and therapeutic efficacy.We used N-hydroxy succinimide as an activator to introduce the glycine methyl ester to detect tumor.We got a porphyrin modified by glycine.The affinity between probe and tumor cell entered GLC-82 cells(human glandular lung cancer cell line)can be observed by Confocal Microscope.The toxicity of probe has been identified by MTT Assay.The summary has been gotten that the porphyrins were nontoxic to GLC-82 cells and glycine modified porphyrin has a good affinity with GLC-82 cells under the iRGD function by our experiment.
- Fluorescence probe,
- iRGD,
- Glycine,
- Porphyrin,
- Near-infrared
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