Citation: Huang Zhi-Xiang, Xie Qin, Guo Qiu-Ping, Wang Ke-Min, Meng Xiang-Xian, Yuan Bao-Yin, Wan Jun, Chen Yuan-Yuan. DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues[J]. Chinese Chemical Letters, ;2017, 28(6): 1252-1257. doi: 10.1016/j.cclet.2017.01.002 shu

DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China

Corresponding author: Guo Qiu-Ping, guoqping@126.com Wang Ke-Min, kmwang@hnu.edu.cn
Received Date: 13 October 2016
Revised Date: 13 December 2016
Accepted Date: 13 December 2016
Available Online: 7 June 2017

Figures(4)

Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics, calling for effective molecular probes. Herein, we performed cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) to obtain specific recognition of human prostate cancer cells PC-3M. Four aptamers were successfully obtained that can bind to target cells with high affinity and specificity. A 51-nt truncated sequence named Xq-2-C1 was identified after further elaborative analysis on the secondary structure. More importantly, the achieved aptamer Xq-2-C1 not only demonstrated excellent specific to target cells, but also revealed specific recognition to clinical prostate cancer tissue. The tissue imaging results showed that Xq-2-C1 had better recognition ratio for clinical prostate cancer tissue samples (85%) compared to the random sequence (9%). These results demonstrate that these newly generated aptamers would furnish potential applications in the early diagnosis and clinical treatment of prostate cancer.
- Aptamer,
- Cell-SELEX,
- Prostate cancer,
- PC-3M,
- Clinical tissues
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