Citation: Hou Guang-Yue, Men Li-Hui, Wang Lu, Zheng Zhong, Liu Zhi-Qiang, Song Feng-Rui, Liu Zhong-Ying. Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats[J]. Chinese Chemical Letters, ;2017, 28(6): 1214-1219. doi: 10.1016/j.cclet.2016.12.039 shu

Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats

a.
National Center for Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China

Corresponding author: Song Feng-Rui, songfr@ciac.ac.cn

Received Date: 30 August 2016
Revised Date: 17 November 2016
Accepted Date: 1 December 2016
Available Online: 8 June 2017

Figures(5)

The effect of Radix Scutellariae treated on type 2 diabetic rats has been investigated by a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) based urinary quantitative approach. In this research, multiple reactions monitoring mode of MS/MS in LC-MS/MS analysis was used to quantitatively analyze the concentrations of 7 endogenous compounds in urine of normal control group, type 2 diabetic model group and Radix Scutellariae-treated group, and multivariate statistical analysis was utilized for MS data processing. The above-mentioned three groups can be distinguished via pattern recognition. The obtained results indicated that Radix Scutellariae affect the urinary metabolic profiling of type 2 diabetic rats on the polyol pathway, protein glycation reaction and amino acids metabolism pathway. According to these results, Radix Scutellariae should have the pharmacological effect on preventing or delaying the onset and progression of diabetes and its complications.
- LC-MS/MS,
- Endogenous markers,
- Multivariate statistical analysis,
- Type 2 diabetic complications,
- Radix Scutellariae
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