Citation: Xu Cai-Na, Tian Hua-Yu, Wang Yan-Bing, Du Yang, Chen Jie, Lin Lin, Guo Zhao-Pei, Chen Xue-Si. Anti-tumor effects of combined doxorubicin and siRNA for pulmonary delivery[J]. Chinese Chemical Letters, ;2017, 28(4): 807-812. doi: 10.1016/j.cclet.2016.12.013 shu

Anti-tumor effects of combined doxorubicin and siRNA for pulmonary delivery

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: Tian Hua-Yu, thy@ciac.ac.cn Chen Xue-Si, xschen@ciac.ac.cn
Received Date: 30 August 2016
Revised Date: 8 November 2016
Accepted Date: 24 November 2016
Available Online: 15 April 2016

Figures(7)

Direct administration of drugs and genes to the lungs by pulmonary delivery offers a potential effective therapy for lung cancers.In this study, combined doxorubicin(DOX)and Bcl2 siRNA was employed for cancer therapy using polyethylenimine(PEI)as the carrier of Bcl2 siRNA.Most of the DOX and siRNA possessed high cellular uptake efficiency in B16F10 cells, which was proved by FCM and CLSM analysis. Real-time PCR showed that PEI/Bcl2 siRNA exhibited high gene silencing efficiency with 70% Bcl2 mRNA being knocked down.The combination of DOX and siRNA could enhance the cell proliferation inhibition and the cell apoptosis against B16F10 cells compared to free DOX or PEI/Bcl2 siRNA.Furthermore, the biodistribution of DOX and siRNA via pulmonary administration was studied in mice with B16F10 metastatic lung cancer.The results showed that most of the DOX and siRNA were accumulated in lungs and lasted at least for 3 days, which suggested that combined DOX and siRNA by pulmonary administration may have high anti-tumor effects for metastatic lung cancer treatment in vivo.
- Pulmonary delivery,
- Anti-tumor effects,
- Metastatic lung cancer,
- Combined DOX and siRNA therapy,
- Synergistic anti-tumor
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