Citation: Zhou Chunhua, A.Abdel-Rahman Mona, Li Wen, Liu Kun, Zhang Afang. Thermoresponsive dendronized copolymers for protein recognitions based on biotin-avidin interaction[J]. Chinese Chemical Letters, ;2017, 28(4): 832-838. doi: 10.1016/j.cclet.2016.11.016 shu

Thermoresponsive dendronized copolymers for protein recognitions based on biotin-avidin interaction

a.
Department of Polymer Materials, Shanghai University, 99 Shangda Road, Mailbox 152, Shanghai 200444, China
b.
Chemistry Department, Polymer Lab.109, Faculty of Science, Assiut University, PB 71516, Assiut, Egypt

Corresponding author: Zhang Afang, azhang@shu.edu.cn
Received Date: 19 October 2016
Revised Date: 6 November 2016
Accepted Date: 8 November 2016
Available Online: 18 April 2016

Figures(6)

Thermoresponsive biotinylated dendronized copolymers carrying dendritic oligoethylene glycol(OEG) pendants were prepared via free radical polymerization, and their protein recognitions based on biotin-avidin interaction investigated.Both first(PG1) and second generation(PG2) dendronized copolymers were designed to examine possible thickness effects on the interaction between biotin and avidin.Inherited from the outstanding thermoresponsive properties from OEG dendrons, these biotinylated cylindrical copolymers show characteristic thermoresponsive behavior which provides an envelope to capture avidin through switching temperatures above or below their phase transition temperatures(T_cps).Thus, the recognition of polymer-supported biotin with avidin was investigated with UV/vis spectroscopy and dynamic laser light scattering.In contrast to the case for PG1, the increased thickness for copolymer PG2 hinders partially and inhibits the recognition of biotin moieties with avidin either below or above its T_cp.This demonstrates the significant architecture effects from dendronized polymers on the biotin moieties to shift onto periphery of the collapsed aggregates, which should be a prerequisite for protein recognition.These kinds of novel thermoresponsive copolymers may pave a way for the interesting biological applications in areas such as reversible activity control of enzyme or proteins, and for controlled delivery of drugs or genes.
- Dendronized copolymers,
- Dendrimers,
- Thermoresponsive polymers,
- Protein recognition,
- Biotin-avidin interaction,
- Supramolecular chemistry
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1.
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