Citation: Xiao-Xu Ma, Cui-Cui Wang, Wen-Sheng Cai, Xue-Guang Shao. Quantification of albumin in urine using preconcentration and near-infrared diffuse reflectance spectroscopy[J]. Chinese Chemical Letters, ;2016, 27(10): 1597-1601. doi: 10.1016/j.cclet.2016.03.008 shu

Quantification of albumin in urine using preconcentration and near-infrared diffuse reflectance spectroscopy

a.
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China;
b.
Tianjin Key Laboratory of Biosensing and Molecular Recognition, Tianjin 300071, China;
c.
State Key Laboratory of Medicinal Chemical Biology, Tianjin 300071, China
d.
Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300071, China

Corresponding author: Xue-Guang Shao, xshao@nankai.edu.cn
Received Date: 1 February 2016
Revised Date: 1 March 2016
Accepted Date: 4 March 2016
Available Online: 16 October 2016

Figures(3)

Urinary albumin is an important diagnostic and prognostic marker for cardiorenal disease. Recent studies have shown that elevation of albumin excretion even in normal concentration range is associated with increased cardiorenal risk. Therefore, accurate measurement of urinary albumin in normal concentration range is necessary for clinical diagnosis. In this work, thiourea-functionalized silica nanoparticles are prepared and used for preconcentration of albumin in urine. The adsorbent with the analyte was then used for near-infrared diffuse reflectance spectroscopy measurement directly and partial least squares model was established for quantitative prediction. Forty samples were taken as calibration set for establishing PLS model and 17 samples were used for validation of the method. The correlation coefficient and the root mean squared error of cross validation is 0.9986 and 0.43, respectively. Residual predictive deviation value of the model is as high as 18.8. The recoveries of the 17 validation samples in the concentration range of 3.39-24.39 mg/L are between 95.9%-113.1%. Therefore, the method may provide a candidate method to quantify albumin excretion in urine.
- Near-infrared diffuse reflectance,
- spectroscopy,
- Quantitative determination,
- Multivariate calibration,
- Urinary albumin,
- Thiourea-functionalized silica,
- nanoparticles
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