Citation: Mishaal Mohammed Ahmed. Fabrication and characterization of gold nano particles for DNA biosensor applications[J]. Chinese Chemical Letters, ;2016, 27(5): 801-806. doi: 10.1016/j.cclet.2016.01.013 shu

Fabrication and characterization of gold nano particles for DNA biosensor applications

Mishaal Mohammed Ahmed ^a,b,,

a.
Chemistry Department, College of Science, Anbar University, Ramadi, Iraq
b.
Institutes of Nano Electronic Engineering, University Malaysia Perlis, 01000 Kangar, Perlis, Malaysia

Corresponding author: Mishaal Mohammed Ahmed, mishal78_2010@yahoo.com
Received Date: 5 September 2015
Revised Date: 27 November 2015
Accepted Date: 5 January 2016
Available Online: 14 May 2016

Figures(8)

This research involves the preparation of a biosensor using silicon oxide for biomedical applications, and its effective use for the detection of target DNA hybridization. An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl) tri-ethoxysilane (APTES) as a linker molecule combined with gold nanoparticles (GNPs) on a thermally oxidized SiO₂ thin film. The size of the GNPs was calculated by utilizing UV-vis data with an average calculated particle size within the range of 30±5 nm, and characterization by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The GNP-modified SiO₂ thin films were electrically characterized through the measurement of capacitance, permittivity and conductivity using a low-cost dielectric analyzer. The capacitance, permittivity and conductivity profiles of the fabricated sensor clearly differentiated DNA immobilization and hybridization.
- Gold nanoparticles,
- Electrochemical,
- DNA hybridization,
- Dielectric analyzer
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