Citation: Ying-Hui Ma, Meng-Ying Wei, Yuan-Yuan Liu, Feng-Rui Song, Zhong-Ying Liu, Zi-Feng Pi. Study on intestinal transport of Veratrum alkaloids compatible with Panax ginseng across the Caco-2 cell monolayer model by UPLC-ESI-MS method[J]. Chinese Chemical Letters, ;2016, 27(02): 215-220. doi: 10.1016/j.cclet.2015.10.003 shu

Study on intestinal transport of Veratrum alkaloids compatible with Panax ginseng across the Caco-2 cell monolayer model by UPLC-ESI-MS method

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a. College of Pharmacy, Jilin University, Changchun 130021, China;
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b. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: Zhong-Ying Liu, Zi-Feng Pi,
Received Date: 25 May 2015
Available Online: 7 July 2015
Fund Project: This research was supported by the National Natural Science Foundation of China (Nos. 81073040, 81274046, 81173507) (Nos. 81073040, 81274046, 81173507) the National Basic Research Program of China (National 973 Program) (Nos. 2011CB505300, 2011CB505305) (National 973 Program)

The Caco-2 cells have been recognized as effective tools to be applied to imitate the drug absorption in human intestine for the transport of drug. In this study, Caco-2 cell monolayer model was used to study compatibility of the transport of the Veratrum alkaloids in different proportions with Panax ginseng. A specific ultra-high performance liquid chromatographic-electrospray ionization-mass spectrometric (UPLC-ESI-MS) method is developed for the semi-quantitative determination of Veratrum alkaloids on intestinal transport with berberine as internal standard (IS). In the Caco-2 model constructed, three influencing factors are investigated, including time, concentration and recovery rates of the Veratrum alkaloids during the uptake from AP (apical side) to BL (basolateral side). The results suggest that the flux of Veratrum alkaloids is time dependent and concentration dependent. And the absorption of all eight Veratrum alkaloids increase after compatibility with Panax ginseng compared to the single Veratrum nigrum extraction. This research was studied from the perspective of intestinal absorption by the UPLCESI-MSmethod. Thismethod was successfully applied to transport studies of the Veratrum alkaloids and the interaction mechanism between Veratrum nigrum and Panax ginseng.
- UPLC-ESI-MS,
- Veratrum nigrum,
- Alkaloids,
- Panax ginseng,
- Transport,
- Caco-2 cell monolayer model
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