Citation: Yan Deng, Liang Qiao, Natalia Gasilova, Xin-Xiang Zhang, Hubert H. Girault. Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization[J]. Chinese Chemical Letters, ;2016, 27(01): 85-87. doi: 10.1016/j.cclet.2015.09.017 shu

Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization

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a Beijing National Laboratory for Molecular Sciences(BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China;
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b Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Switzerland

Corresponding author: Xin-Xiang Zhang, Hubert H. Girault,
Received Date: 22 April 2015
Available Online: 2 June 2015

The coupling between open channel-based microchip electrophoresis and mass spectrometry via electrostatic spray ionization is proposed for in situ detection of fractionated analytes. Electrophoretic separation is performed in an open channel fabricated in a plastic substrate. The solvent of background electrolyte is evaporated from the open channel because of Joule heating during electrophoresis, leaving the dried electrophoretic bands to be directly analyzed by mass spectrometry via scanning electrostatic spray ionization. Proof-of-concept results are obtained with fluorescent dyes and antibiotics as the test samples, demonstrating an efficient on-chip detection platform based on the electrophoresis and electrostatic spray ionization mass spectrometry.
- Open channel,
- Microchip electrophoresis,
- Interface,
- Electrostatic spray ionization mass,
- spectrometry
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