Citation: Zhi Ping Zhang, Yi Ming Li, Xiao Yun Chen, Qing Xiang Guo. Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group[J]. Chinese Chemical Letters, ;2011, 22(3): 338-341. doi: 10.1016/j.cclet.2010.10.007 shu

Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group

a Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;
b Department of Chemistry, Tsinghua University, Beijing 100084, China

Received Date: 25 June 2010

Fund Project: This study was supported by the National Natural Science Foundation of China (No. 90713009).

Green fluorescent protein (GFP) plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore (BHQ) for controlling its expression with exact spatiotemporal resolution. In vitro and in vivo experiments clearly verified that, comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.
- Photoregulation,
- Caging(uncaging),
- BHQ,
- Green fluorescent protein plasmid,
- Photolysis
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