Citation: Xue Ping Ji, Xian Rui Li, Na Wang, Rui Xing Ni, Xiao Hong Liu, Hua Ai Xiong. Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor[J]. Chinese Chemical Letters, ;2010, 21(10): 1239-1242. doi: 10.1016/j.cclet.2010.05.022 shu

Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor

Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China

Received Date: 23 February 2010

Fund Project: This work was financially supported by the Scientific Research Foundation of State Education Ministry of China (Jiaowaisiliu[2008]890) and Research Foundation of Education Department of Hebei Province of China (No.2007132).

A mixed self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic acid amide (T-NH₂) were used to immobilize tyrosinase for fabricating biosensor. The results showed that the mixed SAMs prepared from solution at the ratio of 1:4provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate. The biosensor exhibited a fast response and high sensitivity for sensing substrate.
- Biosensor,
- Tyrosinase,
- Catechol,
- Self-assembled monolayers,
- Gold electrode
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