Citation: Jian She Tang, Li Xiang. Development of a competitive format sorbent assay for the determination of parathion in water using molecular imprinted polymer as specific sorbent carrier[J]. Chinese Chemical Letters, ;2010, 21(11): 1361-1365. doi: 10.1016/j.cclet.2010.05.014 shu

Development of a competitive format sorbent assay for the determination of parathion in water using molecular imprinted polymer as specific sorbent carrier

Jian She Tang ,
Li Xiang

National Experimental Teaching Demonstration Laboratory of Water Pollution Control and Recovery Utilization from Wastewater, Anhui University of Architecture, Hefei 230022, China

Received Date: 4 March 2010

Fund Project: This work was supported by the Excellent Young Teacher Fund of Anhui Province, China (No.2009SQRZ105) and the Dr.Fund of Anhui University of Architecture (No.QD20090905).

A rapid, simple, and reliable competitive molecular imprinted polymer sorbent assay (MIPSA) was developed and validated for measurement of parathion in water samples. This assay employed a molecular imprinted polymer (MIP) that was synthesized with non-covalent imprinting method as capture reagent and p-aminoparathion conjugate of horseradish peroxidase(para-HRP)as an enzyme label. The assay depended on a competitive binding reaction between the enzyme conjugate and analyte for the binding sites of the MIE The optimized analysis conditions of 10 ng mL^-1 para-HRP and 10 mg mL^-1 polymer were found. The assay was acceptable to detect parathion in water samples under the optimized conditions, with a limit of detection of 50 ng mL^-1. Mean analytical recoveries of added parathion in water samples ranged from 101.2% to 105%. The precision of the assay was satisfactory;relative standard deviation ranged from 4.3% to 6%.
- Molecular imprinting,
- Conjugated enzyme,
- Sorbent assay,
- Parathion,
- Water sample
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