Citation: Li Da Han, Qiong Lin Liang, Yi Ming Wang, Ping Hu, Guo An Luo. A new metabonomics method for simultaneous determination of EFAs and NEFAs in plasma using GC-MS and its application[J]. Chinese Chemical Letters, ;2009, 20(9): 1103-1106. doi: 10.1016/j.cclet.2009.04.022 shu

A new metabonomics method for simultaneous determination of EFAs and NEFAs in plasma using GC-MS and its application

Li Da Han ^a,b, ,
Qiong Lin Liang ^b ,
Yi Ming Wang ^b ,
Ping Hu ^a ,
Guo An Luo ^a,b

a School of Pharmaceutics, East-China University of Science & Technology, Shanghai 200237, China;
b Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China

Received Date: 29 December 2008

Fund Project: China).The studies are supported by grant from the National Basic Research Development Program of China (973 Programhe samples are offered by China-Japan Friendship Hospital (BeijingNo.2005CB523503)and from the National Natural Science Foundations of China (Nos.90709045 and 20805026).

A new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids (EFAs and NEFAs) in plasma. Merely 10 μL of plasma was required. The pretreatment of the sample was simple without disposing the protein. After simple extraction and derivation, 15 FAs in plasma were precisely quantified. Gas chromatography tandem mass spectrometry (GC-MS) was used in the study and the quantities of the analytes, which varied in abundance over three orders of magnitude. The established method showed good accuracy. The curve correlation coefficients were all above 0.997. The RSDs of precision for all compounds were below 15% and recoveries were all between 80 and 110%. PLS-DA modal was used in the data management.b
- Metabonomics,
- Fatty acids,
- Quantification,
- GC-MS,
- PLS-DA
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