Citation: Wen Ting An, Yong Jiao, Xiao Hua Sun, Chuan Dong, Shao Min Shuang, Ping Fang Xia, Man Shing Wong. Protein surface recognition of the novel tetra-carboxylphenyl calix[4]arene to cytochrome c[J]. Chinese Chemical Letters, ;2008, 19(11): 1341-1344. doi: 10.1016/j.cclet.2008.09.004 shu

Protein surface recognition of the novel tetra-carboxylphenyl calix[4]arene to cytochrome c

a Research Center of Environmental Science and Engineering, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
b Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hongkong S.A.R., China

Received Date: 28 April 2008

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 90813018),the Youth Foundation of Shanxi Province (No. 2006021009),as well as the Youth Scientific and Technical Foundation of Shanxi University (Nos. 2006007 and 2007112).

The interaction of the novel tetra-carboxylphenyl calix[4]arene (TCPC) with the bovine heart cytochrome c (Cc) was first investigated by fluorescence spectroscopy and molecular modeling methods.The formation of a stable 1:1 complex was monitored by fluorescence titration,and its binding constant is 1.916×10⁷ L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface,that is,the electrostatic interaction drives TCPC to the Cc surface,and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.
- Tetra-carboxylphenyl calix[4]arene,
- Cytochrome c,
- Protein surface recognition,
- Fluorescence spectroscopy,
- Molecular modeling
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