Citation: Shu Ping Zhang, Lian Gang Shan, Zhen Ran Tian, Yi Zheng, Li Yi Shi, Deng Song Zhang. Study of enzyme biosensor based on carbon nanotubes modified electrode for detection of pesticides residue[J]. Chinese Chemical Letters, ;2008, 19(5): 592-594. doi: 10.1016/j.cclet.2008.03.014 shu

Study of enzyme biosensor based on carbon nanotubes modified electrode for detection of pesticides residue

a College of Science, University of Shanghai for Science and Technology, Shanghai 200093, China;
b School of Material Science and Engineering, Shanghai University, Shanghai 200072, China;
c Jiangsu Food Science College, Jiangsu 223003, China;
d Nano-science Technology Center, Shanghai University, Shanghai 200444, China

Received Date: 19 November 2007

he paper describes a controllable layer-by-layer (LBL) self-assembly modification technique of multi-walled carbon nanotubes(MWNTs) and poly(diallyldimethylammonium chloride) (PDDA) towards glassy carbon electrode (GCE), Acetylcholinesterase(ACHE) was immobilized directly to the modified GCE by LBL self-assembly method, the activity value of AChE was detected by using i-t technique based on the modified Ellman method. Then the composition of carbaryl were detected by the enzyme electrode with 0.01U activity value and the detection limit of carbaryl is 10^-12 g L^-1 so the enzyme biosensor showed good properties for pesticides residue detection.
- Acetylcholinesterase,
- Carbon nanotubes modified electrode,
- Layer-by-layer self-assembly,
- Immobilization
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