Citation:
Min Chen, Si Hui Li, Li Sun. A novel phycocyanin-Chla/c2-protein complex isolated from chloroplasts of Chroomonas placoidea[J]. Chinese Chemical Letters,
;2007, 18(11): 1374-1378.
doi:
10.1016/j.cclet.2007.09.025
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Nine pigment-protein complexes were separated and characterized from intact Chroomonas placoidea chloroplasts by IEF. The bands Ⅰ-Ⅵ with their isoelectric points (pI) values from 4 to 6 were phycocyanin components; bands Ⅷ and Ⅸ (pI=2.8-3.6) were chlorophyll-protein complexes. According to absorption and fluorescence spectra, band Ⅶ was designated as a novel phycocyanin-Chla/c2-protein complex (pI≈3.4-3.7). These results indicated that phycocyanin is structurally and functionally coupled with chlorophyll-protein complex in C. placoidea, and probably interacted with electrostatic force in combination.
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Keywords:
- Chroomonas placoidea,
- IEF,
- Chlorophyll-protein complex,
- Phycocyanin
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