Citation: Jian Quan Lu, Ming Bo Xu, Xing Wang Zhou, Jin Guang Xu, Qing Tao. A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy[J]. Chinese Chemical Letters, ;2007, 18(4): 441-444. doi: 10.1016/j.cclet.2007.01.012 shu

A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy

a Hubei Key Laboratory of Bioanalytical Technique, Hubei Normal University, Huangshi 435002, China;
b Department of Chemistry, Central China Normal University, Wuhan 430079, China

Received Date: 13 October 2006

Fund Project: This work was partially supported by the Science Foundation of the National Education Ministry (No. 206096) and the Education Department of Hubei Province (No. Z200522002).

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit (S/N=3) of 0.07 ng/mL.
- Detection,
- Single-stranded DNA binding protein,
- ss-DNA,
- Surface plasmon resonance microscopy
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