Citation: Zhen Hu, Hai Ping Xing, Zhou Zhu, Wei Wang, Wen Chao Guan. Synthesis of cystine C₆₀ derivative and its protective effects on hydrogen peroxide-induced apoptosis in PC12 cells[J]. Chinese Chemical Letters, ;2007, 18(2): 145-148. doi: 10.1016/j.cclet.2006.12.023 shu

Synthesis of cystine C₆₀ derivative and its protective effects on hydrogen peroxide-induced apoptosis in PC12 cells

a Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, China;
b Department of Neurology, Tongji Hospital, Tongji Medical College, Wuhan 430030, China

Received Date: 10 October 2006

Fund Project: The project is supported by the National Natural Science Foundation of China (No. 20474020).

Oxidative stress has been considered as a major cause of cellular injuries in a variety of clinical abnormalities, especially prominent in neural diseases. One of the usable ways to prevent the reactive oxygen species (ROS)-mediated cellular injury is dietary or pharmaceutical augmentation of some free radical scavenger. Water-soluble amino-fullerene is a novel compound that behaves as a free radical scavenger with excellent biology consistent. In the present study, we have synthesized and characterized a novel cystine C₆₀ derivative for the first time, and investigated the effects on hydrogen peroxide-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. PC12 cells treated with hydrogen peroxide underwent apoptotic death as determined by MTT, PI/Hoechst 33342 staining and flow cytometry analysis. These results suggested that cystine C₆₀ derivative has the potential to prevent oxidative stress-induced cell death and has no evident toxicity.
- Cystine C₆₀derivative,
- Reactive oxygen species,
- H₂O₂,
- Apoptosis,
- PC12 cells
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