Citation: De Bin Zhu, Da Xing, Ya Bing Tang. Highly sensitive ECL-PCR method for detection of K-ras point mutation[J]. Chinese Chemical Letters, ;2007, 18(2): 198-200. doi: 10.1016/j.cclet.2006.12.017 shu

Highly sensitive ECL-PCR method for detection of K-ras point mutation

MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510635, China

Received Date: 7 July 2006

Fund Project: 60378043 30470494),and the Natural Science Foundation of Guangdong Province (Nos. 015012This research is supported by the National Natural Science Foundation of China (Nos. 30600128 2004B10401011). 04010394

A highly sensitive electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for K-ras point mutation detection is developed. Briefly, K-ras oncogene was amplified by a Ru(bpy)₃²⁺ (TBR)-labeled forward and a biotin-labeled reverse primer,and followed by digestion with MvaI restriction enzyme, which only cut the wild-type amplicon containing its cutting site. The digested product was then adsorbed to the streptavidin-coated microbead through the biotin label and detected by ECL assay. The experiment results showed that the different genotypes can be clearly discriminated by ECL-PCR method. It is useful in point mutation detection, due to its sensitivity, safety, and simplicity.
- Electrochemiluminescence-polymerase chain reaction,
- K-ras oncogene,
- Point mutation detection
1. [1]
  Rui Wang , Yang Liang , Julius Rebek Jr. , Yang Yu . Stabilization and detection of labile reaction intermediates in supramolecular containers. Chinese Chemical Letters, 2024, 35(6): 109228-. doi: 10.1016/j.cclet.2023.109228
2. [2]
  Xianghua Zeng , Weichen Meng , Xiaochun Han , Jiachen Yang , Kaiqi Wu , Fengxian Gao , Xiliang Luo . Highly stable and antifouling solid-contact ion-selective electrode for K⁺ detection in complex system based on multifunctional peptide and conductive MOF. Chinese Chemical Letters, 2025, 36(8): 110564-. doi: 10.1016/j.cclet.2024.110564
3. [3]
  Zhuangzhuang Zhang , Yaru Qiao , Jun Zhao , Dai-Huo Liu , Mengmin Jia , Hongwei Tang , Liang Wang , Dongmei Dai , Bao Li . Fluorine-doped K_0.39Mn_0.77Ni_0.23O_1.9F_0.1 microspheres with highly reversible oxygen redox reaction for potassium-ion battery cathode. Chinese Chemical Letters, 2025, 36(3): 109907-. doi: 10.1016/j.cclet.2024.109907
4. [4]
  Quanxing Mao , Zhengliang Wang , Zhinan Hu , Ziqi Yang , Hui Li , Yali Yao , Zijun Yong , Tianyi Ma . Facial detection of formaldehyde by using acidichromic carbon dots and the reaction between formaldehyde and ammonium chloride. Chinese Chemical Letters, 2025, 36(7): 110499-. doi: 10.1016/j.cclet.2024.110499
5. [5]
  Zhaorui Song , Qiulian Hao , Bing Li , Yuwei Yuan , Shanshan Zhang , Yongkuan Suo , Hai-Hao Han , Zhen Cheng . NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers. Chinese Chemical Letters, 2025, 36(1): 109834-. doi: 10.1016/j.cclet.2024.109834
6. [6]
  Shaoqiang Wen , Lang Zhang , Zhan Huang , Meng Chen , Yuzhi Xu , Xing Huang , Zhou Zhang , Xiaoyong Zou , Zong Dai , Si-Yang Liu . Aptamer&MOF-functionalized multichannel paper chip for point-of-care testing of small extracellular vesicle membrane protein profile. Chinese Chemical Letters, 2026, 37(2): 111333-. doi: 10.1016/j.cclet.2025.111333
7. [7]
  Jian Han , Li-Li Zeng , Qin-Yu Fei , Yan-Xiang Ge , Rong-Hui Huang , Fen-Er Chen . Recent advances in remote C(sp³)–H functionalization via chelating group-assisted metal-catalyzed chain-walking reaction. Chinese Chemical Letters, 2024, 35(11): 109647-. doi: 10.1016/j.cclet.2024.109647
8. [8]
  Yuyang Zhou , Ziwang Mao , Jing-Juan Xu . Recent advances in near infrared (NIR) electrochemiluminescence luminophores. Chinese Chemical Letters, 2024, 35(11): 109622-. doi: 10.1016/j.cclet.2024.109622
9. [9]
  Sijia Zhou , Tianyi Zhou , Yuhua Hou , Wang Li , Yanfei Shen , Songqin Liu , Kaiqing Wu , Yuanjian Zhang . Recent advances in electrochemiluminescence based on polymeric luminophores. Chinese Chemical Letters, 2025, 36(5): 110284-. doi: 10.1016/j.cclet.2024.110284
10. [10]
  Zimo Yang , Yan Tong , Yongbo Liu , Qianlong Liu , Zhihao Ni , Yuna He , Yu Rao . Developing selective PI3K degraders to modulate both kinase and non-kinase functions. Chinese Chemical Letters, 2024, 35(11): 109577-. doi: 10.1016/j.cclet.2024.109577
11. [11]
  Wang CHEN , Guixian WU , Yingying XIONG , Ming CHENG , Mingming ZHOU , Hui ZHANG , Zhengyun LIANG , Dejin HUANG , Minghua CHEN . Crystal structures of complexes of cyclobutanocucurbit[5]uril with Na⁺/K⁺: Coordination state in different anionic environments. Chinese Journal of Inorganic Chemistry, 2026, 42(1): 161-169. doi: 10.11862/CJIC.20250195
12. [12]
  Zihong Li , Jie Cheng , Ping Huang , Guoliang Wu , Weiying Lin . Activatable photoacoustic bioprobe for visual detection of aging in vivo. Chinese Chemical Letters, 2024, 35(4): 109153-. doi: 10.1016/j.cclet.2023.109153
13. [13]
  Chong Wang , Hao Xie , Rulan Xia , Xuewei Liao , Jin Wang , Huajun Yang , Chen Wang . Nanofluidic ion rectification sensor for enantioselective recognition and detection. Chinese Chemical Letters, 2025, 36(8): 110642-. doi: 10.1016/j.cclet.2024.110642
14. [14]
  Qihang Wu , Hui Wen , Wenhai Lin , Tingting Sun , Zhigang Xie . Alkyl chain engineering of boron dipyrromethenes for efficient photodynamic antibacterial treatment. Chinese Chemical Letters, 2024, 35(12): 109692-. doi: 10.1016/j.cclet.2024.109692
15. [15]
  Huiju Cao , Lei Shi . sp¹-Hybridized linear and cyclic carbon chain. Chinese Chemical Letters, 2025, 36(4): 110466-. doi: 10.1016/j.cclet.2024.110466
16. [16]
  Juan Chen , Jin-Ni Zhao , Guo-Yu Yang . Four zinc borates: From acentric chain to centric porous layer. Chinese Journal of Structural Chemistry, 2026, 45(2): 100788-100788. doi: 10.1016/j.cjsc.2025.100788
17. [17]
  Xiafeng Yang , Wushan Sun , Chen Li , Wei Bai , Qingyin Wang , Gongying Wang . Synthesis of novel isosorbide-based polycarbonates by melt chain extension method. Chinese Chemical Letters, 2026, 37(4): 111674-. doi: 10.1016/j.cclet.2025.111674
18. [18]
  Bo-Ran Chang , Lin Deng , Qing-Lian Wu , Wan-Qian Guo , Hui-Ying Xue . A review: Carbon-based materials as effective additives in anaerobic fermentation, focusing on microbial chain elongation and medium chain fatty acids production. Chinese Chemical Letters, 2025, 36(7): 110411-. doi: 10.1016/j.cclet.2024.110411
19. [19]
  Miao-Miao Chen , Min-Ling Zhang , Xiao Song , Jun Jiang , Xiaoqian Tang , Qi Zhang , Xiuhua Zhang , Peiwu Li . Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples. Chinese Chemical Letters, 2025, 36(1): 109785-. doi: 10.1016/j.cclet.2024.109785
20. [20]
  Kun Zou , Yihang Xiao , Jinyu Yang , Mingxuan Wu . Facile semisynthesis of histone H3 enables nucleosome probes for investigation of histone H3K79 modifications. Chinese Chemical Letters, 2024, 35(10): 109497-. doi: 10.1016/j.cclet.2024.109497

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1385)
HTML views(65)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return