Citation: Wangcheng Zhan, Yongjun Lü, Ling Yang, Yanglong Guo, Yanqin Wang, Yun Guo, Guanzhong Lu. Epoxidation of vinyl functionalized cubic Ia3d mesoporous silica for immobilization of penicillin G acylase[J]. Chinese Journal of Catalysis, ;2014, 35(10): 1709-1715. doi: 10.1016/S1872-2067(14)60156-X shu

Epoxidation of vinyl functionalized cubic Ia3d mesoporous silica for immobilization of penicillin G acylase

1.
Key Laboratory for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: Guanzhong Lu,
Received Date: 24 March 2014
Available Online: 15 April 2014
Fund Project:

Epoxy functionalized cubic Ia3d mesoporous silica (CIMS) was successfully synthesized by epoxidizing vinyl groups prepared on the CIMS by a co-condensation method. The synthesized material was characterized by X-ray diffraction, nitrogen sorption, transmission electron microscopy, thermogravimetric analysis, and solid state ¹³C NMR. The vinyl groups were found to be easily epoxidized to yield epoxy functionalized CIMS. The epoxy functionalized CIMS was used as a support for the immobilization of penicillin G acylase (PGA), and the effects of the epoxy group on the initial activity and the operational stability of the immobilized PGA were studied. The results showed that the enzyme loading and initial activity of the immobilized PGA decreased with increasing amounts of epoxy groups. These observations were due to a decrease in the pore size of the mesoporous silica as well as an increase in the hydrophobicity of the silica surface. However, an appropriate amount of epoxy groups on the CIMS support was found to improve the operational stability of the immobilized PGA. This improvement was the result of increased interactions between the epoxy functionalized CIMS support and the PGA.
- Co-condensation method,
- Mesoporous silica,
- Enzyme,
- Immobilization,
- Penicillin G acylase
1. [1]
  [1] Bornscheuer U T. Angew Chem Int Ed, 2003, 42: 3336
2. [2]
  [2] DiCosimo R, McAuliffe J, Poulose A J, Bohlmann G. Chem Soc Rev, 2013, 42: 6437
3. [3]
  [3] Katchalski-Katzir E, Kraemer D M. J Mol Catal B, 2000, 10: 157
4. [4]
  [4] Cao L Q. Curr Opin Chem Biol, 2005, 9: 217
5. [5]
  [5] An Z, He J, Lu S, Yang L. AIChE J, 2010, 56: 2677
6. [6]
  [6] Liese A, Hilterhaus L. Chem Soc Rev, 2013, 42: 6236
7. [7]
  [7] Liu X H, Bu C H, Nan Z H, Zheng L C, Qiu Y, Lu X Q. Talanta, 2013, 105: 63
8. [8]
  [8] He J, Song Z H, Ma H, Yang L, Guo C X. J Mater Chem, 2006, 16: 4307
9. [9]
  [9] Yiu H H P, Wright P A. J Mater Chem, 2005, 15: 3690
10. [10]
  [10] Hartmann M. Chem Mater, 2005, 17: 4577
11. [11]
  [11] On D T, Desplantier-Giscard D, Danumah C, Kaliaguine S. Appl Catal A, 2001, 222: 299
12. [12]
  [12] Hartmann M, Jung D. J Mater Chem, 2010, 20: 844
13. [13]
  [13] Kim J, Grate J W, Wang P. Chem Eng Sci, 2006, 61: 1017
14. [14]
  [14] Chong A S M, Zhao X S. Appl Surf Sci, 2004, 237: 398
15. [15]
  [15] Yiu H H P, Wright P A, Botting N P. J Mol Catal B, 2001, 15: 81
16. [16]
  [16] Wight A P, Davis M E. Chem Rev, 2002, 102: 3589
17. [17]
  [17] Ispas C, Sokolov I, Andreescu S. Anal Boianal Chem, 2009, 393: 543
18. [18]
  [18] Stein A, Melde B J, Schroden R C. Adv Mater, 2000, 12: 1403
19. [19]
  [19] Burkett S L, Sims S D, Mann S. Chem Commun, 1996: 1367
20. [20]
  [20] Macquarrie D J. Chem Commun, 1996: 1961
21. [21]
  [21] Hoffmann F, Cornelius M, Morell J, Froba M. Angew Chem Int Ed, 2006, 45: 3216
22. [22]
  [22] Lim M H, Stein A. Chem Mater, 1999, 11: 3285
23. [23]
  [23] Sakaguchi K, Matsui M, Mizukami F. Appl Microbiol Biotechnol, 2005, 67: 306
24. [24]
  [24] Miyahara M, Vinu A, Ariga K. J Nanosci Nanotechnol, 2006, 6: 1765
25. [25]
  [25] Sheldon R A, van Pelt S. Chem Soc Rev, 2013, 42: 6223
26. [26]
  [26] Sayari A, Hamoudi S. Chem Mater, 2001, 13: 3151
27. [27]
  [27] Huh S, Wiench J W, Yoo J C, Pruski M, Lin V S Y. Chem Mater, 2003, 15: 4247
28. [28]
  [28] MacLachlan M J, Asefa T, Ozin G A. Chem-Eur J, 2000, 6: 2507
29. [29]
  [29] Wang J, Zou Y C, Sun Y, Hemgesberg M, Shffner D, Gao H C, Song X J, Zhang W X, Jia M J, Thiel W R. Chin J Catal (王静, 邹永存, 孙渝, Hemgeserg M, Shffner D, 高洪成, 宋晓静, 张文祥, 贾明君, Thiel W R. 催化学报), 2014, 35: 532
30. [30]
  [30] Cai W J, Zhou Y, Bao R L, Yue B, He H Y. Chin J Catal (蔡雯佳, 周琰, 包任烈, 岳斌, 贺鹤勇. 催化学报), 2013, 34: 193
31. [31]
  [31] Richer R. Chem Comm, 1998: 1775
32. [32]
  [32] Hoffmann F, Cornelius M, Morell J, Froba M. J Nanosci Nanotechnol, 2006, 6: 265
33. [33]
  [33] Mateo C, Fernandez-Lorente G, Abian O, Fernandez-Lafuente R, Guisan J M. Biomacromolecules, 2000, 1: 739
34. [34]
  [34] Boller T, Meier C, Menzler S. Org Process Res Dev, 2002, 6: 509
35. [35]
  [35] Lü Y J, Lu G Z, Wang Y Q, Guo Y L, Guo Y, Zhang Z G, Wang Y S, Liu X H. Adv Funct Mater, 2007, 17: 2160
36. [36]
  [36] Xue P, Xu F, Xu L D. Appl Surf Sci, 2008, 255: 1625
37. [37]
  [37] Wang Y Q, Zibrowius B, Yang C M, Spliethoff B, Schüth F. Chem Commun, 2004: 46
38. [38]
  [38] Wang Y Q, Yang C M, Zibrowius B, Spliethoff B, Linden M, Schüth F. Chem Mater, 2003, 15: 5029
39. [39]
  [39] Xia L Y, Zhang M Q, Rong M Z, Xu W M. Phys Chem Chem Phys, 2010, 12: 10569
40. [40]
  [40] Schwartz N N, Blumbergs J H. J Org Chem, 1964, 29: 1976
41. [41]
  [41] Asefa T, Kruk M, MacLachlan M J, Coombs N, Grondey H, Jaroniec M, Ozin G A. Adv Funct Mater, 2001, 11: 447
42. [42]
  [42] Pang J B, Hampsey J E, Hu Q Y, Wu Z W, John V T, Lu Y F. Chem Commun, 2004: 682
43. [43]
  [43] Ryoo R, Joo S H, Kim J M. J Phys Chem B, 1999, 103: 7435
44. [44]
  [44] Kleitz F, Choi S H, Ryoo R. Chem Commun, 2003: 2136
45. [45]
  [45] Xue P, Lu G Z, Guo Y L, Wang Y S, Guo Y. J Mol Catal B, 2004, 30: 75
46. [46]
  [46] Yang C M, Zibrowius B, Schüth F. Chem Commun, 2003: 1772
47. [47]
  [47] Yang C M, Zibrowius B, Schmidt W, Schüth F. Chem Mater, 2004, 16: 2918
48. [48]
  [48] Yang C M, Smatt J H, Zibrowius B, Linden M. New J Chem, 2004, 28: 1520
49. [49]
  [49] Webb P A, Orr C. Analytical Methods in Fine Particle Technology. Norcross: Micromertics Instrument Corporation, 1997. 53
50. [50]
  [50] Shimada T, Aoki K, Shinoda Y, Nakamura T, Tokunaga N, Inagaki S, Hayashi T. J Am Chem Soc, 2003, 125: 4688
51. [51]
  [51] Zhao X S, Lu G Q. J Phys Chem B, 1998, 102: 1556
1. [1]
  Peiling Li , Qing Feng , Hongling Yuan , Qin Wang . Live Interview Recording about the Penicillin Family. University Chemistry, 2024, 39(9): 122-127. doi: 10.3866/PKU.DXHX202311022
2. [2]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
3. [3]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
4. [4]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
5. [5]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
6. [6]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
7. [7]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
8. [8]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
9. [9]
  Pei Li , Yuenan Zheng , Zhankai Liu , An-Hui Lu . Boron-Containing MFI Zeolite: Microstructure Control and Its Performance of Propane Oxidative Dehydrogenation. Acta Physico-Chimica Sinica, 2025, 41(4): 100034-. doi: 10.3866/PKU.WHXB202406012
10. [10]
  Jiali CHEN , Guoxiang ZHAO , Yayu YAN , Wanting XIA , Qiaohong LI , Jian ZHANG . Machine learning exploring the adsorption of electronic gases on zeolite molecular sieves. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 155-164. doi: 10.11862/CJIC.20240408
11. [11]
  Yiping HUANG , Liqin TANG , Yufan JI , Cheng CHEN , Shuangtao LI , Jingjing HUANG , Xuechao GAO , Xuehong GU . Hollow fiber NaA zeolite membrane for deep dehydration of ethanol solvent by vapor permeation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 225-234. doi: 10.11862/CJIC.20240224
12. [12]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
13. [13]
  Shanghua Li , Malin Li , Xiwen Chi , Xin Yin , Zhaodi Luo , Jihong Yu . 基于高离子迁移动力学的取向ZnQ分子筛保护层实现高稳定水系锌金属负极的构筑. Acta Physico-Chimica Sinica, 2025, 41(1): 2309003-. doi: 10.3866/PKU.WHXB202309003
14. [14]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
15. [15]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
16. [16]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
17. [17]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
18. [18]
  Lijun Huo , Mingcun Wang , Tianyi Zhao , Mingjie Liu . Exploration of Undergraduate and Graduate Integrated Teaching in Polymer Chemistry with Aerospace Characteristics. University Chemistry, 2024, 39(6): 103-111. doi: 10.3866/PKU.DXHX202312059
19. [19]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
20. [20]
  Hongling Yuan , Jialin Xie , Jiawei Wang , Jixiang Zhao , Jiayan Liu , Qing Feng , Wei Qi , Min Liu . Cyclic Olefin Copolymer (COC): The Agile Vanguard in the Realm of Materials. University Chemistry, 2024, 39(7): 294-298. doi: 10.12461/PKU.DXHX202311041

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(689)
HTML views(52)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142