Citation: Bo Yang, Jian-Zhong Du. Ultrasound-responsive Homopolymer Nanoparticles[J]. Chinese Journal of Polymer Science, ;2020, 38(4): 349-356. doi: 10.1007/s10118-020-2345-6 shu

Ultrasound-responsive Homopolymer Nanoparticles

Bo Yang ^a,b ,
Jian-Zhong Du ^a,b,,

a.
Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
b.
Department of Polymeric Materials, School of Materials Science and Engineering, Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Tongji University, Shanghai 201804, China

Corresponding author: Jian-Zhong Du, jzdu@tongji.edu.cn
Received Date: 25 July 2019
Revised Date: 22 August 2019
Available Online: 24 September 2019

Noninvasive ultrasound is a more effective strategy for on-demand drug delivery of polymeric nanoparticles than many other stimuli. However, the preparation of ultrasound-responsive homopolymer nanoparticles is still very challenging. In this study, we disclose the regulating factors of ultrasound responsiveness of homopolymer nanoparticles and the disaggregation behavior of homopolymer nanoparticle aggregates. Homopolymer nanoparticles such as vesicles and large compound micelles (LCMs) are self-assembled from poly(methoxyethyl methacrylate) (PMEMA) and poly(amic acid) (PAA), respectively. The ultrasound responsiveness of PAA vesicles at metastable state could be regulated by tuning the self-assembly temperature (T_s), and was optimized when T_s is around the glass transition temperature (T_g) of PAA. However, the PMEMA LCMs did not respond to ultrasound as they are at stable state. On the other hand, poly(2-(2-ethoxyethoxy)ethyl acrylate) (PEEA) could self-assemble into vesicle aggregates or complex micelle aggregates, which were dissociated upon sonication. Overall, the above findings provide us with a fresh insight for designing ultrasound-responsive polymeric nanoparticles.
- Ultrasound responsiveness,
- Homopolymers,
- Vesicles,
- Micelles,
- Self-assembly
1. [1]
  Mai, Y.; Eisenberg, A. Self-assembly of block copolymers. Chem. Soc. Rev. 2012, 41, 5969−5985. doi: 10.1039/c2cs35115c
2. [2]
  Wang, M. Z.; Wang, T.; Yuan, K.; Du, J. Z. Preparation of water dispersible poly(methyl methacrylate)-based vesicles for facile persistent antibacterial applications. Chinese J. Polym. Sci. 2016, 34, 44−51. doi: 10.1007/s10118-016-1725-4
3. [3]
  Zou, Y. J.; He, S. S.; Du, J. Z. ε-Poly(L-lysine)-based hydrogels with fast-acting and prolonged antibacterial activities. Chinese J. Polym. Sci. 2018, 36, 1239−1250. doi: 10.1007/s10118-018-2156-1
4. [4]
  Xiao, J. G.; Hu, Y.; Du, J. Z. Polymer nanodisks by collapse of nanocapsules. Sci. China Chem. 2018, 61, 569−575. doi: 10.1007/s11426-017-9209-3
5. [5]
  Song, T.; Xi, Y. J.; Du, J. Z. Antibacterial hydrogels incorporated with poly(glutamic acid)-based vesicles. Acta Polymerica Sinica (in Chinese) 2018, 119−128. doi: 10.11777/j.issn1000-3304.2018.17229
6. [6]
  Peer, D.; Karp, J. M.; Hong, S.; Farokhzad, O. C.; Margalit, R.; Langer, R. Nanocarriers as an emerging platform for cancer therapy. Nat. Nanotechnol. 2007, 2, 751−760. doi: 10.1038/nnano.2007.387
7. [7]
  Zhu, Y. Q.; Yang, B.; Chen, S.; Du, J. Z. Polymer vesicles: Mechanism, preparation, application, and responsive behavior. Prog. Polym. Sci. 2017, 64, 1−22. doi: 10.1016/j.progpolymsci.2015.05.001
8. [8]
  Chen, W. Q.; Du, J. Z. Ultrasound and pH dually responsive polymer vesicles for anticancer drug delivery. Sci. Rep. 2013, 3, 2162. doi: 10.1038/srep02162
9. [9]
  Zhao, Y. Z.; Du, L. N.; Lu, C. T.; Jin, Y. G.; Ge, S. P. Potential and problems in ultrasound-responsive drug delivery systems. Int. J. Nanomed. 2013, 8, 1621−1633.
10. [10]
  Wang, D. R.; Wang, X. G. Amphiphilic azo polymers: molecular engineering, self-assembly and photoresponsive properties. Prog. Polym. Sci. 2013, 38, 271−301. doi: 10.1016/j.progpolymsci.2012.07.003
11. [11]
  Al-Ahmady, Z.; Kostarelos, K. Chemical components for the design of temperature-responsive vesicles as cancer therapeutics. Chem. Rev. 2016, 116, 3883−3918. doi: 10.1021/acs.chemrev.5b00578
12. [12]
  Yuan, K.; Zhou, X.; Du, J. Z. Synthesis and characterization of thermo-responsive polypeptide-based vesicles with photo-cross-linked membranes. Acta Phys. Chim. Sin. 2017, 33, 656−660.
13. [13]
  Wang, F. Y. K.; Gao, J. Y.; Xiao, J. G.; Du, J. Z. Dually gated polymersomes for gene delivery. Nano Lett. 2018, 18, 5562−5568. doi: 10.1021/acs.nanolett.8b01985
14. [14]
  Xu, X. F.; Pan, C. Y.; Zhang, W. J.; Hong, C. Y. Polymerization-induced self-assembly generating vesicles with adjustable pH-responsive release performance. Macromolecules 2019, 52, 1965−1975. doi: 10.1021/acs.macromol.9b00144
15. [15]
  Qiu, L.; Zhao, L.; Xing, C.; Zhan, Y. Redox-responsive polymer prodrug/AgNPs hybrid nanoparticles for drug delivery. Chin. Chem. Lett. 2018, 29, 301−304. doi: 10.1016/j.cclet.2017.09.048
16. [16]
  Tan, J.; Deng, Z.; Liu, G.; Hu, J.; Liu, S. Anti-inflammatory polymersomes of redox-responsive polyprodrug amphiphiles with inflammation-triggered indomethacin release characteristics. Biomaterials 2018, 178, 608−619. doi: 10.1016/j.biomaterials.2018.03.035
17. [17]
  Mo, R.; Jiang, T.; Di, J.; Tai, W.; Gu, Z. Emerging micro- and nanotechnology based synthetic approaches for insulin delivery. Chem. Soc. Rev. 2014, 43, 3595−3629. doi: 10.1039/c3cs60436e
18. [18]
  Xiao, Y. F.; Hu, Y.; Du, J. Z. Controlling blood sugar levels with a glycopolymersome. Mater. Horiz. 2019, DOI: 10.1039/C9MH00625G. doi: 10.1039/C9MH00625G
19. [19]
  Wright, D. B.; Thompson, M. P.; Touve, M. A.; Carlini, A. S.; Gianneschi, N. C. Enzyme-responsive polymer nanoparticles via ring-opening metathesis polymerization-induced self-assembly. Macromol. Rapid Commun. 2019, 40, 1800467. doi: 10.1002/marc.201800467
20. [20]
  Xuan, J.; Pelletier, M.; Xia, H.; Zhao, Y. Ultrasound-induced disruption of amphiphilic block copolymer micelles. Macromol. Chem. Phys. 2011, 212, 498−506. doi: 10.1002/macp.201000624
21. [21]
  Xuan, J.; Boissiere, O.; Zhao, Y.; Yan, B.; Tremblay, L.; Lacelle, S.; Xia, H.; Zhao, Y. Ultrasound-responsive block copolymer micelles based on a new amplification mechanism. Langmuir 2012, 28, 16463−16468. doi: 10.1021/la303946b
22. [22]
  Yin, T.; Wang, P.; Li, J.; Zheng, R.; Zheng, B.; Cheng, D.; Li, R.; Lai, J.; Shuai, X. Ultrasound-sensitive siRNA-loaded nanobubbles formed by hetero-assembly of polymeric micelles and liposomes and their therapeutic effect in gliomas. Biomaterials 2013, 34, 4532−4543. doi: 10.1016/j.biomaterials.2013.02.067
23. [23]
  Yin, T.; Wang, P.; Li, J.; Wang, Y.; Zheng, B.; Zheng, R.; Cheng, D.; Shuai, X. Tumor-penetrating codelivery of siRNA and paclitaxel with ultrasound-responsive nanobubbles hetero-assembled from polymeric micelles and liposomes. Biomaterials 2014, 35, 5932−5943. doi: 10.1016/j.biomaterials.2014.03.072
24. [24]
  Wang, Y.; Yin, T.; Su, Z.; Qiu, C.; Wang, Y.; Zheng, R.; Chen, M.; Shuai, X. Highly uniform ultrasound-sensitive nanospheres produced by a pH-induced micelle-to-vesicle transition for tumor-targeted drug delivery. Nano Res. 2018, 11, 3710−3721. doi: 10.1007/s12274-017-1939-y
25. [25]
  Zhang, L.; Yin, T.; Li, B.; Zheng, R.; Qiu, C.; Lam, K. S.; Zhang, Q.; Shuai, X. Size-modulable nanoprobe for high-performance ultrasound imaging and drug delivery against cancer. ACS Nano 2018, 12, 3449−3460. doi: 10.1021/acsnano.8b00076
26. [26]
  Zhou, F.; Xie, M.; Chen, D. Structure and ultrasonic sensitivity of the superparticles formed by self-assembly of single chain Janus nanoparticles. Macromolecules 2014, 47, 365−372. doi: 10.1021/ma401589z
27. [27]
  Zhang, J.; Liu, K.; Mullen, K.; Yin, M. Self-assemblies of amphiphilic homopolymers: synthesis, morphology studies and biomedical applications. Chem. Commun. 2015, 51, 11541−11555. doi: 10.1039/C5CC03016A
28. [28]
  Zhu, Y. Q.; Fan, L.; Yang, B.; Du, J. Z. Multifunctional homopolymer vesicles for facile immobilization of gold nanoparticles and effective water remediation. ACS Nano 2014, 8, 5022−31. doi: 10.1021/nn5010974
29. [29]
  Fan, L.; Lu, H.; Zou, K. D.; Chen, J.; Du, J. Z. Homopolymer vesicles with a gradient bilayer membrane as drug carriers. Chem. Commun. 2013, 49, 11521−11523. doi: 10.1039/c3cc45873c
30. [30]
  Sun, H.; Liu, D. Q.; Du, J. Z. Nanobowls with controlled openings and interior holes driven by the synergy of hydrogen bonding and π-π interaction. Chem. Sci. 2019, 10, 657−664. doi: 10.1039/C8SC03995J
31. [31]
  Sun, H.; Zhu, Y. Q.; Yang, B.; Wang, Y. F.; Wu, Y. P.; Du, J. Z. Template-free fabrication of nitrogen-doped hollow carbon spheres for high-performance supercapacitors based on a scalable homopolymer vesicle. J. Mater. Chem. A 2016, 4, 12088−12097. doi: 10.1039/C6TA04330E
32. [32]
  Zhu, Y. Q.; Liu, L.; Du, J. Z. Probing into homopolymer self-assembly: How does hydrogen bonding influence morphology? Macromolecules 2013, 46, 194−203. doi: 10.1021/ma302176a
33. [33]
  Sun, H.; Du, J. Z. Plasmonic vesicles with tailored collective properties. Nanoscale 2018, 10, 17354−17361. doi: 10.1039/C8NR04820G
34. [34]
  Liu, J.; Huang, W.; Pang, Y.; Huang, P.; Zhu, X.; Zhou, Y.; Yan, D. Molecular self-assembly of a homopolymer: an alternative to fabricate drug-delivery platforms for cancer therapy. Angew. Chem. Int. Ed. 2011, 50, 9162−9166. doi: 10.1002/anie.201102280
35. [35]
  Yin, M.; Kuhlmann, C. R. W.; Sorokina, K.; Li, C.; Mihov, G.; Pietrowski, E.; Koynov, K.; Klapper, M.; Luhmann, H. J.; Müllen, K.; Weil, T. Novel fluorescent core-shell nanocontainers for cell membrane transport. Biomacromolecules 2008, 9, 1381−1389. doi: 10.1021/bm701138g
36. [36]
  Yin, M.; Shen, J.; Pflugfelder, G. O.; Müllen, K. A fluorescent core-shell dendritic macromolecule specifically stains the extracellular matrix. J. Am. Chem. Soc. 2008, 130, 7806−7807. doi: 10.1021/ja8022362
37. [37]
  Sandanaraj, B. S.; Demont, R.; Thayumanavan, S. Generating patterns for sensing using a single receptor scaffold. J. Am. Chem. Soc. 2007, 129, 3506−3507. doi: 10.1021/ja070229f
38. [38]
  Mane, S. R.; Rao N, V.; Chaterjee, K.; Dinda, H.; Nag, S.; Kishore, A.; Das Sarma, J.; Shunmugam, R. Amphiphilic homopolymer vesicles as unique nano-carriers for cancer therapy. Macromolecules 2012, 45, 8037−8042. doi: 10.1021/ma301644m
39. [39]
  Lim, E. K.; Huh, Y. M.; Yang, J.; Lee, K.; Suh, J. S.; Haam, S. pH-triggered drug-releasing magnetic nanoparticles for cancer therapy guided by molecular imaging by MRI. Adv. Mater. 2011, 23, 2436−2442. doi: 10.1002/adma.201100351
40. [40]
  Sun, H.; Wang, F. Y. K.; Du, J. Z. Preparation, application and perspective in polymer vesicles with an inhomogeneous membrane. Sci. Sin. Chim. 2019, 49, 877−890. doi: 10.1360/N032018-00259
1. [1]
  Yuanpeng Ye , Longfei Yao , Guofeng Liu . Engineering circularly polarized luminescence through symmetry manipulation in achiral tetraphenylpyrazine structures. Chinese Journal of Structural Chemistry, 2025, 44(2): 100460-100460. doi: 10.1016/j.cjsc.2024.100460
2. [2]
  Sifan Du , Yuan Wang , Fulin Wang , Tianyu Wang , Li Zhang , Minghua Liu . Evolution of hollow nanosphere to microtube in the self-assembly of chiral dansyl derivatives and inversed circularly polarized luminescence. Chinese Chemical Letters, 2024, 35(7): 109256-. doi: 10.1016/j.cclet.2023.109256
3. [3]
  Yuwen Zhu , Xiang Deng , Yan Wu , Baode Shen , Lingyu Hang , Yuye Xue , Hailong Yuan . Formation mechanism of herpetrione self-assembled nanoparticles based on pH-driven method. Chinese Chemical Letters, 2025, 36(1): 109733-. doi: 10.1016/j.cclet.2024.109733
4. [4]
  Jingqi Xin , Shupeng Han , Meichen Zheng , Chenfeng Xu , Zhongxi Huang , Bin Wang , Changmin Yu , Feifei An , Yu Ren . A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance. Chinese Chemical Letters, 2024, 35(7): 109165-. doi: 10.1016/j.cclet.2023.109165
5. [5]
  Keyang Li , Yanan Wang , Yatao Xu , Guohua Shi , Sixian Wei , Xue Zhang , Baomei Zhang , Qiang Jia , Huanhua Xu , Liangmin Yu , Jun Wu , Zhiyu He . Flash nanocomplexation (FNC): A new microvolume mixing method for nanomedicine formulation. Chinese Chemical Letters, 2024, 35(10): 109511-. doi: 10.1016/j.cclet.2024.109511
6. [6]
  Xuanyu Wang , Zhao Gao , Wei Tian . Supramolecular confinement effect enabling light-harvesting system for photocatalytic α-oxyamination reaction. Chinese Chemical Letters, 2024, 35(11): 109757-. doi: 10.1016/j.cclet.2024.109757
7. [7]
  Xian Yan , Huawei Xie , Gao Wu , Fang-Xing Xiao . Boosted solar water oxidation steered by atomically precise alloy nanocluster. Chinese Chemical Letters, 2025, 36(1): 110279-. doi: 10.1016/j.cclet.2024.110279
8. [8]
  Feng Cao , Chunxiang Xian , Tianqi Yang , Yue Zhang , Haifeng Chen , Xinping He , Xukun Qian , Shenghui Shen , Yang Xia , Wenkui Zhang , Xinhui Xia . Gelation-pyrolysis strategy for fabrication of advanced carbon/sulfur cathodes for lithium-sulfur batteries. Chinese Chemical Letters, 2025, 36(3): 110575-. doi: 10.1016/j.cclet.2024.110575
9. [9]
  Zhenzhu Wang , Chenglong Liu , Yunpeng Ge , Wencan Li , Chenyang Zhang , Bing Yang , Shizhong Mao , Zeyuan Dong . Differentiated self-assembly through orthogonal noncovalent interactions towards the synthesis of two-dimensional woven supramolecular polymers. Chinese Chemical Letters, 2024, 35(5): 109127-. doi: 10.1016/j.cclet.2023.109127
10. [10]
  Cheng-Yan Wu , Yi-Nan Gao , Zi-Han Zhang , Rui Liu , Quan Tang , Zhong-Lin Lu . Enhancing self-assembly efficiency of macrocyclic compound into nanotubes by introducing double peptide linkages. Chinese Chemical Letters, 2024, 35(11): 109649-. doi: 10.1016/j.cclet.2024.109649
11. [11]
  Xiaofei NIU , Ke WANG , Fengyan SONG , Shuyan YU . Self-assembly of [Pd₆(L)₄]⁸⁺-type macrocyclic complexes for fluorescent sensing of HSO₃^-. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1233-1242. doi: 10.11862/CJIC.20240057
12. [12]
  Zengchao Guo , Weiwei Liu , Tengfei Liu , Jinpeng Wang , Hui Jiang , Xiaohui Liu , Yossi Weizmann , Xuemei Wang . Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs. Chinese Chemical Letters, 2024, 35(7): 109060-. doi: 10.1016/j.cclet.2023.109060
13. [13]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
14. [14]
  Ruoxi Sun , Yiqian Xu , Shaoru Rong , Chunmiao Han , Hui Xu . The Enchanting Collision of Light and Time Magic: Exploring the Footprints of Long Afterglow Lifetime. University Chemistry, 2024, 39(5): 90-97. doi: 10.3866/PKU.DXHX202310001
15. [15]
  Changhui Yu , Peng Shang , Huihui Hu , Yuening Zhang , Xujin Qin , Linyu Han , Caihe Liu , Xiaohan Liu , Minghua Liu , Yuan Guo , Zhen Zhang . Evolution of template-assisted two-dimensional porphyrin chiral grating structure by directed self-assembly using chiral second harmonic generation microscopy. Chinese Chemical Letters, 2024, 35(10): 109805-. doi: 10.1016/j.cclet.2024.109805
16. [16]
  Bing Niu , Honggao Huang , Liwei Luo , Li Zhang , Jianbo Tan . Coating colloidal particles with a well-defined polymer layer by surface-initiated photoinduced polymerization-induced self-assembly and the subsequent seeded polymerization. Chinese Chemical Letters, 2025, 36(2): 110431-. doi: 10.1016/j.cclet.2024.110431
17. [17]
  Yi Zhou , Wei Zhang , Rong Fu , Jiaxin Dong , Yuxuan Liu , Zihang Song , Han Han , Kang Cai . Self-assembly of two pairs of homochiral M₂L₄ coordination capsules with varied confined space using Tröger's base ligands. Chinese Chemical Letters, 2025, 36(2): 109865-. doi: 10.1016/j.cclet.2024.109865
18. [18]
  Tong Zhang , Chao Sun , Shubin Yang , Zimin Cai , Sifeng Zhu , Wendian Liu , Yun Luan , Cheng Wang . Inhalation of taraxasterol loaded mixed micelles for the treatment of idiopathic pulmonary fibrosis. Chinese Chemical Letters, 2024, 35(8): 109248-. doi: 10.1016/j.cclet.2023.109248
19. [19]
  Mengjuan Sun , Muye Zhou , Yifang Xiao , Hailei Tang , Jinhua Chen , Ruitao Zhang , Chunjiayu Li , Qi Ya , Qian Chen , Jiasheng Tu , Qiyue Wang , Chunmeng Sun . Reversibly size-switchable polyion complex micelles for antiangiogenic cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109110-. doi: 10.1016/j.cclet.2023.109110
20. [20]
  Gaojian Yang , Zhiyang Li , Rabia Usman , Zhu Chen , Yuan Liu , Song Li , Hui Chen , Yan Deng , Yile Fang , Nongyue He . DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer. Chinese Chemical Letters, 2025, 36(2): 109930-. doi: 10.1016/j.cclet.2024.109930

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(4400)
HTML views(149)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142