Citation: Xu-Dong Shi, Pei-Jian Sun, Zhi-Hua Gan. Preparation of Porous Polylactide Microspheres and Their Application in Tissue Engineering[J]. Chinese Journal of Polymer Science, ;2018, 36(6): 712-719. doi: 10.1007/s10118-018-2079-x shu

Preparation of Porous Polylactide Microspheres and Their Application in Tissue Engineering

Xu-Dong Shi ^a,b ,
Pei-Jian Sun ^b ,
Zhi-Hua Gan ^b,c,,

a.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
The CAS Key Laboratory of Engineering Plastics, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, China
c.
State Key Laboratory of Organic-inorganic Composites, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

Corresponding author: Zhi-Hua Gan, zhgan@mail.buct.edu.cn
Received Date: 2 September 2017
Accepted Date: 26 October 2017
Available Online: 19 January 2018

In this study, porous polylactide (PLA) microspheres with different structures were prepared through the multiple emulsion solvent evaporation method. By changing organic solvents (ethyl acetate and chloroform) and adding effervescent salt NH₄HCO₃ in the inner water phase, microspheres with porous capsular, matrix, microcapsular and multivesicular structures were prepared. The protein encapsulation and release, and the cell growth behavior of porous microspheres were further explored. Under the same inner water phase, microspheres prepared with chloroform had higher protein encapsulation efficiency and less protein release rate as compared with those prepared with ethyl acetate. Cell experiments showed that the relatively rough surface of microspheres prepared with chloroform was more favorable for the cell growth in comparison with the smooth surface of microspheres prepared with ethyl acetate. This study shows a simple and effective method to control the protein release and cell growth behaviors of polymer microspheres by tuning their porous structure.
- Polylactide,
- Porous microspheres,
- Drug release,
- Cell,
- Tissue engineering
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