Citation: Ni-jia Song, Li-juan Zhou, Wen-kai Liu, Xue-ling He, Zhi-cheng Pan, Ming-ming Ding, Xin-yuan Wan, Jie-hua Li, Hong Tan, Feng Luo, Qiang Fu. Effect of Trastuzumab on the Micellization Properties, Endocytic Pathways and Antitumor Activities of Polyurethane-based Drug Delivery System[J]. Chinese Journal of Polymer Science, ;2017, 35(8): 909-923. doi: 10.1007/s10118-017-1952-3 shu

Effect of Trastuzumab on the Micellization Properties, Endocytic Pathways and Antitumor Activities of Polyurethane-based Drug Delivery System

Ni-jia Song ^a,† ,
Li-juan Zhou ^a,b,† ,
Wen-kai Liu ^a ,
Xue-ling He ^a,c ,
Zhi-cheng Pan ^a ,
Ming-ming Ding ^a ,
Xin-yuan Wan ^a ,
Jie-hua Li ^a,, ,
Hong Tan ^a,, ,
Feng Luo ^a ,
Qiang Fu ^a

a.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China
b.
Key Laboratory of Rubber-Plastics, Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao University of Science & Technology, Qingdao 266042, China
c.
Laboratory Animal Center of Sichuan University, Huaxi Clinical College, Sichuan University, Chengdu 610040, China

Corresponding author: Jie-hua Li, jiehua_li@scu.edu.cn Hong Tan, hongtan@scu.edu.cn

^†

Received Date: 12 February 2017
Revised Date: 13 March 2017
Accepted Date: 13 March 2017

Fund Project: the National Science Fund for Distinguished Young Scholars of China 51425305the Youth Science and Technology Innovation Team of Sichuan Province 2015TD0001the National Natural Science Foundation of China 51273126the National Natural Science Foundation of China 51573112

Polyurethane micelles (PM)-based nanovehicles have shown great potential in targeted delivery of therapeutics and diagnostics into tumors. However, the pathways of PMs entering cancer cells and the action mechanism of targeting ligands have yet to be understood. In this contribution, the actively-targeted PM were developed using trastuzumab as a model targeting group. It was found that PM were mainly taken up by SKOV-3 tumor cells via a micropinocytosis process, while the incorporation of trastuzumab to PM enabled a receptor-mediated endocytosis of nanocarriers in cancer cells, leading to more efficient cell entry and enhanced anticancer efficacy of chemotherapeutic drugs both in vitro and in vivo. This study is advantageous to the understanding of the action mechanism of trastuzumab, and significant for the construction of improved formulations for targeted delivery and precise therapy.
- Polyurethane,
- Micelle,
- Trastuzumab,
- Endocytic pathway,
- Antitumor
1. [1]
  Hanahan, D. and Weinberg, R., Cell, 2011, 144(5):646 doi: 10.1016/j.cell.2011.02.013
2. [2]
  Holohan, C., van Schaeybroeck, S., Longley, D.B. and Johnston, P.G., Nat. Rev. Cancer., 2013, 13(10):714 doi: 10.1038/nrc3599
3. [3]
  Yachida, S., Jones, S., Bozic, I., Antal, T., Leary, R., Fu, B., Kamiyama, M., Hruban, R.H., Eshleman, J.R. and Nowak, M.A., Nature, 2010, 467(7319):1114 doi: 10.1038/nature09515
4. [4]
  Vogelstein, B., Papadopoulos, N., Velculescu, V.E., Zhou, S., Diaz, L.A. and Kinzler, K.W., Science, 2013, 339(6127):1546 doi: 10.1126/science.1235122
5. [5]
  Shi, J., Kantoff, P.W., Wooster, R. and Farokhzad, O.C., Nat. Rev. Cancer, 2017, 17(1):20
6. [6]
  Gu, L. and Mooney, D.J., Nat. Rev. Cancer, 2015, 16(1):56 doi: 10.1038/nrc.2015.3
7. [7]
  Saleh, T. and Shojaosadati, S.A., Hum. Vacc. Immunother., 2016, 12(7):1863
8. [8]
  Asadi, N., Davaran, S., Panahi, Y., Hasanzadeh, A., Malakootikhah, J., Fallah, Moafi, H. and Akbarzadeh, A., Artif. Cell Nanomed. Biotechnol., 2017, 45(1):18 doi: 10.1080/21691401.2016.1178136
9. [9]
  Zheng, Y., Stephan, M.T., Gai, S.A., Abraham, W., Shearer, A. and Irvine, D.J., J. Control. Release, 2011, 172(2):426
10. [10]
  Noh, Y.W., Kim, S.Y., Kim, J.E., Kim, S., Ryu, J., Kim, I., Lee, E., Um, S.H. and Lim, Y.T., Adv. Funct. Mater., 2017, DOI:10.1002/adfm.201605398 doi: 10.1002/adfm.201605398
11. [11]
  Koshy, S.T., Cheung, A.S., Gu, L., Graveline, A.R., and Mooney, D.J., Adv. Biosys., 2017, (1):1600013
12. [12]
  Lv, S., Li, M., Tang, Z., Song, W., Sun, H., Liu, H. and Chen, X., Acta Biomater., 2013, 9(12):9330 doi: 10.1016/j.actbio.2013.08.015
13. [13]
  Torchilin V.P., Nat. Rev. Drug Discov., 2014, 13(11):813 doi: 10.1038/nrd4333
14. [14]
  Yoo, J.W., Chambers, E. and Mitragotri, S., Curr. Pharm. Design., 2010, 16(21):2298 doi: 10.2174/138161210791920496
15. [15]
  Petros, R.A. and Desimone, J.M., Nat. Rev. Drug. Discov., 2010, 9(9):615
16. [16]
  Bertrand, N., Wu, J., Xu, X., Kamaly, N. and Farokhzad, O.C., Adv. Drug. Deliver. Rev., 2014, 66(1):2
17. [17]
  Bazak, R., Houri, M., El Achy, S., Kamel, S. and Refaat, T., J. Cancer Res. Clin. Oncol., 2015, 141(5):769 doi: 10.1007/s00432-014-1767-3
18. [18]
  Mura, S., Nicolas, J. and Couvreur, P., Nat. Mater., 2013, 12(11):991 doi: 10.1038/nmat3776
19. [19]
  Lu, D., Wen, X., Liang, J., Zhang, X., Gu, Z. and Fan, Y., Chinese J. Polym. Sci., 2008, 26(3):369 doi: 10.1142/S0256767908003023
20. [20]
  Yu. S., Ding. J., He, C., Cao, Y., Xu, W. and Chen, X., Adv. Health. Mater., 2014, 3(5):752 doi: 10.1002/adhm.201300308
21. [21]
  Xu, S., Olenyuk, B.Z., Okamoto, C.T. and Hamm-Alvarez, S.F., Adv. Drug. Deliver. Rev., 2013, 65(1):121 doi: 10.1016/j.addr.2012.09.041
22. [22]
  Xu, C., Sui, M., Tang, J. and Shen, Y., Chinese J. Polym. Sci., 2011, 29(3):274 doi: 10.1007/s10118-011-1047-5
23. [23]
  Gao, C., Wang, Y., Zhu, W. and Shen, Z., Chinese J. Polym. Sci., 2014, 32(11):1431 doi: 10.1007/s10118-014-1528-4
24. [24]
  Karimi, M., Ghasemi, A., Zangabad, P.S., Rahighi, R., Basri, S.M.M., Mirshekari, H., Amiri, M., Pishabad, Z.S., Aslani, A., Bozorgomid, M., Ghosh, D., Beyzavi, A., Vaseghi, A., Aref, A.R., Haghani, L., Bahrami, S., Hamblin, M.R., Chem. Soc. Rev., 2016, 45(5):1457 doi: 10.1039/C5CS00798D
25. [25]
  Byrne, J.D., Betancourt, T. and Brannon-Peppas, L., Adv. Drug. Deliver. Rev., 2008, 60(15):1615 doi: 10.1016/j.addr.2008.08.005
26. [26]
  Torchilin V. P. , "Drug delivery" (in English), Springer Berlin Heidelberg, Berlin Heidelberg, 2010, p. 3
27. [27]
  Song, H., He, R., Wang, K., Jing, R., Bao, C., Li, N., Ji, J. and Cui, D., Biomaterials, 2010, 31(8):2302 doi: 10.1016/j.biomaterials.2009.11.067
28. [28]
  He, C., Tang, Z., Tian, H. and Chen, X., Adv. Drug. Deliver. Rev., 2016, 98(1):64
29. [29]
  Wu, X., Chen, J., Wu, M. and Zhao, J.X., Theranostics, 2015, 5(4):322 doi: 10.7150/thno.10257
30. [30]
  Zhu, H., Li, J., Zhang, X.B., Ye, M. and Tan, W., ChemMedChem., 2015, 10(1):39 doi: 10.1002/cmdc.v10.1
31. [31]
  Shuhendler, A.J., Prasad, P., Leung, M., Rauth, A.M., Dacosta, R.S. and Xiao, Y.W., Adv. Healthc. Mater., 2012, 1(5):600 doi: 10.1002/adhm.201200006
32. [32]
  Sun, X., Xu, J., Tang, J., Sui, M. and Shen, Y., Chinese J. Polym. Sci., 2011, 29(4):427 doi: 10.1007/s10118-011-1057-3
33. [33]
  Baselga, J. and Albanell, J., J. Ann. Oncol., 2001, 12(Suppl):S35
34. [34]
  Cameron, D.A. and Stein, S., Nat. Clin. Pract. Oncol., 2008, 5(9):512 doi: 10.1038/ncponc1156
35. [35]
  Nahta, R., Yu, D. and Hung, M.C., Nat. Clin. Pract. Oncol., 2006, 3(5):269 doi: 10.1038/ncponc0509
36. [36]
  Tai, W., Mahato, R., Cheng, K., J. Control. Release, 2010, 146(146):264
37. [37]
  Press, M.F., Cordon-Cardo, C. and Slamon, D.J., Oncogene, 1990, 5(7):953
38. [38]
  Germershaus, O. and Bakowsky, M.U., Bioconjugate Chem., 2006, 17(5):1190 doi: 10.1021/bc0601119
39. [39]
  Liu, Y., Li, K., Liu, B. and Feng, S.S., Biomaterials, 2010, 31(35):9145 doi: 10.1016/j.biomaterials.2010.08.053
40. [40]
  Sun, B., Ranganathan, B. and Feng, S., Biomaterials, 2008, 29(4):475 doi: 10.1016/j.biomaterials.2007.09.038
41. [41]
  Hillaireau, H. and Couvreur, P., Cell. Mol. Life. Sci., 2009, 66(17):2873 doi: 10.1007/s00018-009-0053-z
42. [42]
  Hu, X., Hu, J., Tian, J., Ge, Z., Zhang, G., Luo, K. and Liu, S., J. Am. Chem. Soc., 2013, 135(46):17617 doi: 10.1021/ja409686x
43. [43]
  Valabrega, G., Montemurro, F. and Aglietta, M., Ann. Oncol., 2007, 18(6):977 doi: 10.1093/annonc/mdl475
44. [44]
  Ben-Kasus, T., Schechter, B., Lavi, S., Yarden, Y. and Sela, M., P. Natl. Acad. Sci., 2009, 106(9):3294 doi: 10.1073/pnas.0812059106
45. [45]
  Cuello, M., Ettenberg, S.A., Clark, A.S., Keane, M.M., Posner, R.H., Nau, M.M., Dennis, P.A. and Lipkowitz, S., Cancer Res., 2001, 61(12):4892
46. [46]
  Fiszman, G.L. and Jasnis, M.A., J. Breast Cancer, 2011, 2011:352182
47. [47]
  Hommelgaard, A.M., Lerdrup, M. and van Deurs, B., Mol. Biol. Cell., 2004, 15(4):1557 doi: 10.1091/mbc.E03-08-0596
48. [48]
  Song, N., Ding, M., Pan, Z., Li, J., Zhou, L., Tan, H. and Fu, Q., Biomacromolecules, 2013, 14(12):4407 doi: 10.1021/bm401342t
49. [49]
  Ding, M., Li, J., He, X., Song, N., Tan, H., Zhang, Y., Zhou, L., Gu, Q., Deng, H. and Fu, Q., Adv. Mater., 2012, 24(27):3639 doi: 10.1002/adma.v24.27
50. [50]
  Pan, Z., Ren, Y., Song, N., Song, Y., Li, J., He, X., Luo, F., Tan, H. and Fu, Q., Biomacromolecules, 2016, 17(6):2148 doi: 10.1021/acs.biomac.6b00375
51. [51]
  Ding, M., Song, N., He, X., Li, J., Zhou, L., Tan, H., Fu, Q. and Gu, Q., ACS Nano, 2013, 7(3):1918 doi: 10.1021/nn4002769
52. [52]
  Ding, M., Li, J., Tan, H. and Fu, Q., Soft Matter, 2012, 8(20):5414 doi: 10.1039/c2sm07402h
53. [53]
  Ding, M., Zeng, X., He, X., Li, J., Tan, H. and Fu, Q., Biomacromolecules, 2014, 15(8):2896 doi: 10.1021/bm500506v
54. [54]
  Zhou, L., Liang, D., He, X., Li, J., Tan, H., Li, J., Fu, Q. and Gu, Q., Biomaterials, 2012, 33(9):2734 doi: 10.1016/j.biomaterials.2011.11.009
55. [55]
  Liu, P., Sun, Y., Wang, Q., Sun, Y., Li, H. and Duan, Y., Biomaterials, 2014, 35(2):760 doi: 10.1016/j.biomaterials.2013.10.020
56. [56]
  Agarwal, R., Singh, V., Jurney, P., Shi, L., Sreenivasan, S.V. and Roy, K., P. Natl. Acad. Sci., 2013, 110(43):17247 doi: 10.1073/pnas.1305000110
57. [57]
  Agus, D.B., Bunn, P.A., Franklin, W., Garcia, M. and Ozols, R.F., Semin. Oncol., 2000, 27(6 Suppl 11):92
58. [58]
  Hudis, C.A., New Engl. J. Med., 2007, 357(1):39 doi: 10.1056/NEJMra043186
59. [59]
  Kobayashi, H., Watanabe, R. and Choyke, P.L., Theranostics, 2013, 4(1):81
60. [60]
  Iversen, T.G., Skotland, T. and Sandvig, K., Nano Today, 2011, 6(2):176 doi: 10.1016/j.nantod.2011.02.003
61. [61]
  Pelkmans, L. and Helenius, A., Science, 2002, 296(5567):535 doi: 10.1126/science.1069784
62. [62]
  Pickl, M. and Ries, C.H., Oncogene, 2009, 28(3):461 doi: 10.1038/onc.2008.394
63. [63]
  Cirstoiu-Hapca, A., Bossy-Nobs, L., Buchegger, F., Gurny, R. and Delie, F., Int. J. Pharm., 2007, 331(2):190 doi: 10.1016/j.ijpharm.2006.12.002
64. [64]
  Song, N., Zhou, L., Li, J., Pan, Z., He, X., Tan, H., Wan, X., Li, J., Ran, R. and Fu, Q., Nanoscale, 2016, 8(14):7711 doi: 10.1039/C6NR00859C
65. [65]
  Liang, H.F., Chen, C.T., Chen, S.C., Kulkarni, A.R., Chiu, Y.L., Chen, M.C. and Sung, H.W., Biomaterials, 2006, 27(9):2051 doi: 10.1016/j.biomaterials.2005.10.027
66. [66]
  Alani, A.W.G., Bae, Y., Rao, D.A. and Kwon, G.S., Biomaterials, 2010, 31(7):1765 doi: 10.1016/j.biomaterials.2009.11.038
67. [67]
  Faratian, D., Zweemer, A.J., Nagumo, Y., Sims, A.H., Muir, M., Dodds, M., Mullen, P., Um, I., Kay, C., Hasmann, M., Harrison, D.J. and Langdon, S.P., Clin. Cancer Res., 2011, 17(13):4451 doi: 10.1158/1078-0432.CCR-10-2461
1. [1]
  Huijie An , Chen Yang , Zhihui Jiang , Junjie Yuan , Zhongming Qiu , Longhao Chen , Xin Chen , Mutu Huang , Linlang Huang , Hongju Lin , Biao Cheng , Hongjiang Liu , Zhiqiang Yu . Luminescence-activated Pt(Ⅳ) prodrug for in situ triggerable cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109134-. doi: 10.1016/j.cclet.2023.109134
2. [2]
  Xinyu Liu , Jialin Yang , Zonglin He , Jiaoyan Ai , Lina Song , Baohua Liu . Linear polyurethanes with excellent comprehensive properties from poly(ethylene carbonate) diol. Chinese Chemical Letters, 2025, 36(1): 110236-. doi: 10.1016/j.cclet.2024.110236
3. [3]
  Fangzhou Wang , Wentong Gao , Chenghui Li . A weak but inert hindered urethane bond for high-performance dynamic polyurethane polymers. Chinese Chemical Letters, 2024, 35(5): 109305-. doi: 10.1016/j.cclet.2023.109305
4. [4]
  Jing Zhang , Charles Wang , Yaoyao Zhang , Haining Xia , Yujuan Wang , Kun Ma , Junfeng Wang . Application of magnetotactic bacteria as engineering microrobots: Higher delivery efficiency of antitumor medicine. Chinese Chemical Letters, 2024, 35(10): 109420-. doi: 10.1016/j.cclet.2023.109420
5. [5]
  Di ZHANG , Tianxiang XIE , Xu HE , Wanyu WEI , Qi FAN , Jie QIAO , Gang JIN , Ningbo LI . Construction and antitumor activity of pH/GSH dual-responsive magnetic nanodrug. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 786-796. doi: 10.11862/CJIC.20240329
6. [6]
  Jin Wang , Xiaoyan Pan , Junyu Zhang , Qingqing Zhang , Yanchen Li , Weiwei Guo , Jie Zhang . Active molecule-based theranostic agents for tumor vasculature normalization and antitumor efficacy. Chinese Chemical Letters, 2024, 35(8): 109187-. doi: 10.1016/j.cclet.2023.109187
7. [7]
  Zhaomin Tang , Qian He , Jianren Zhou , Shuang Yan , Li Jiang , Yudong Wang , Chenxing Yao , Huangzhao Wei , Keda Yang , Jiajia Wang . Active-transporting of charge-reversal Cu(Ⅱ)-doped mesoporous silica nanoagents for antitumor chemo/chemodynamic therapy. Chinese Chemical Letters, 2024, 35(7): 109742-. doi: 10.1016/j.cclet.2024.109742
8. [8]
  Hong-Tao Ji , Yu-Han Lu , Yan-Ting Liu , Yu-Lin Huang , Jiang-Feng Tian , Feng Liu , Yan-Yan Zeng , Hai-Yan Yang , Yong-Hong Zhang , Wei-Min He . Nd@C₃N₄-photoredox/chlorine dual catalyzed synthesis and evaluation of antitumor activities of 4-alkylated sulfonyl ketimines. Chinese Chemical Letters, 2025, 36(2): 110568-. doi: 10.1016/j.cclet.2024.110568
9. [9]
  Kunya Wang , Bingyu Liu , Daojiang Yan , Jian Bai , Haibo Yu , Youcai Hu . Full biosynthetic pathway of pyrrolobenzoxazines. Chinese Chemical Letters, 2025, 36(1): 109811-. doi: 10.1016/j.cclet.2024.109811
10. [10]
  Jindian Duan , Xiaojuan Ding , Pui Ying Choy , Binyan Xu , Luchao Li , Hong Qin , Zheng Fang , Fuk Yee Kwong , Kai Guo . Oxidative spirolactonisation for modular access of γ-spirolactones via a radical tandem annulation pathway. Chinese Chemical Letters, 2024, 35(10): 109565-. doi: 10.1016/j.cclet.2024.109565
11. [11]
  Huaran Zhang , Yuting Huang , Yingjie Tang , Dekun Kong , Yi Zou . Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing- and type Ⅲ- polyketide pathway. Chinese Chemical Letters, 2024, 35(7): 108968-. doi: 10.1016/j.cclet.2023.108968
12. [12]
  Ting Li , Xinxin Zheng , Lejing Qu , Yuanyuan Ou , Sai Qiao , Xue Zhao , Yajun Zhang , Xinfeng Zhao , Qian Li . A chromatographic method for pursuing potential GPCR ligands with the capacity to characterize their intrinsic activities of regulating downstream signaling pathway. Chinese Chemical Letters, 2024, 35(10): 109792-. doi: 10.1016/j.cclet.2024.109792
13. [13]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
14. [14]
  Weixu Li , Yuexin Wang , Lin Li , Xinyi Huang , Mengdi Liu , Bo Gui , Xianjun Lang , Cheng Wang . Promoting energy transfer pathway in porphyrin-based sp2 carbon-conjugated covalent organic frameworks for selective photocatalytic oxidation of sulfide. Chinese Journal of Structural Chemistry, 2024, 43(7): 100299-100299. doi: 10.1016/j.cjsc.2024.100299
15. [15]
  Xiongbo Song , Jinwen Xiao , Juan Wu , Li Sun , Long Chen . Decellularized amniotic membrane promotes the anti-inflammatory response of macrophages via PI3K/AKT/HIF-1α pathway. Chinese Chemical Letters, 2025, 36(1): 109844-. doi: 10.1016/j.cclet.2024.109844
16. [16]
  Pengyu Chen , Beibei Chen , Man He , Yuxi Zhou , Lei Lei , Jian Han , Bingsheng Zhou , Ligang Hu , Bin Hu . Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging. Chinese Chemical Letters, 2025, 36(2): 109908-. doi: 10.1016/j.cclet.2024.109908
17. [17]
  Yuanyuan Zeng , Fang Liu , Jun Wang , Bianfei Shao , Tao He , Zhongzheng Xiang , Yan Wang , Shunyao Zhu , Tian Yang , Siting Yu , Changyang Gong , Lei Liu . Fisetin micelles precisely exhibit a radiosensitization effect by inhibiting PDGFRβ/STAT1/STAT3/Bcl-2 signaling pathway in tumor. Chinese Chemical Letters, 2025, 36(2): 109734-. doi: 10.1016/j.cclet.2024.109734
18. [18]
  Xiangdong Lai , Tengfei Liu , Zengchao Guo , Yihan Wang , Jiang Xiao , Qingxiu Xia , Xiaohui Liu , Hui Jiang , Xuemei Wang . In situ formed fluorescent gold nanoclusters inhibit hair follicle regeneration in oxidative stress microenvironment via suppressing NFκB signal pathway. Chinese Chemical Letters, 2025, 36(2): 109762-. doi: 10.1016/j.cclet.2024.109762

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(788)
HTML views(3)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142