Citation: Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, Jing-jing Nie, Mao Peng, Bin-yang Du. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, ;2015, 33(11): 1516-1526. doi: 10.1007/s10118-015-1694-z shu

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

1.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science &
2.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding author: Bin-yang Du,
Received Date: 8 April 2015
Revised Date: 28 April 2015

Fund Project: This work was financially supported by the National Natural Science Foundation of China (Nos. 21274129 and 21322406), the Fundamental Research Funds for the Central Universities (No. 2014XZZX003-21), the third level of 2013 Zhejiang Province 151 Talent Project, and Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.

The novel microgels, poly[di(ethylene glycol) methyl ether methacrylate-co-2-methoxyethyl acrylate] poly(DEGMMA-co-MEA) microgels, were synthesized. The poly(DEGMMA-co-MEA) microgels were thermo-sensitive and exhibited a volume phase transitive temperature (VPTT) of 14-22 ℃. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly(DEGMMA-co-MEA) microgels to lower temperatures. The interfacial interaction of poly(DEGMMA-co-MEA) microgels and three model proteins, namely fibrinogen, bovine serum albumin and lysozyme, was investigated by quartz crystal microbalance (QCM). An injection sequence of microgel-after-protein was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly(DEGMMA-co-MEA) microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly(DEGMMA-co-MEA) microgels were negatively charged in Tris-HCl buffer solution (pH = 7.4), the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state (i.e. at 37 ℃) was observed. Furthermore, the incorporation of MEA might weaken the interaction between poly(DEGMMA-co-MEA) microgels and proteins.
- QCM,
- Poly(DEGMMA-co-MEA) microgels,
- Thermosensitive,
- Proteins,
- Interfacial interaction
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
1. [1]
  Makhloufi Zoulikha , Zhongjian Chen , Jun Wu , Wei He . Approved delivery strategies for biopharmaceuticals. Chinese Chemical Letters, 2025, 36(2): 110225-. doi: 10.1016/j.cclet.2024.110225
2. [2]
  Zhen Dai , Linzhi Tan , Yeyu Su , Kerui Zhao , Yushun Tian , Yu Liu , Tao Liu . Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition. Chinese Chemical Letters, 2024, 35(5): 109121-. doi: 10.1016/j.cclet.2023.109121
3. [3]
  Zhibin Ren , Shan Li , Xiaoying Liu , Guanghao Lv , Lei Chen , Jingli Wang , Xingyi Li , Jiaqing Wang . Penetrating efficiency of supramolecular hydrogel eye drops: Electrostatic interaction surpasses ligand-receptor interaction. Chinese Chemical Letters, 2024, 35(11): 109629-. doi: 10.1016/j.cclet.2024.109629
4. [4]
  Cheng Wang , Ji Wang , Dong Liu , Zhi-Ling Zhang . Advances in virus-host interaction research based on microfluidic platforms. Chinese Chemical Letters, 2024, 35(12): 110302-. doi: 10.1016/j.cclet.2024.110302
5. [5]
  Xiuzheng Deng , Yi Ke , Jiawen Ding , Yingtang Zhou , Hui Huang , Qian Liang , Zhenhui Kang . Construction of ZnO@CDs@Co₃O₄ sandwich heterostructure with multi-interfacial electron-transfer toward enhanced photocatalytic CO₂ reduction. Chinese Chemical Letters, 2024, 35(4): 109064-. doi: 10.1016/j.cclet.2023.109064
6. [6]
  Jinli Chen , Shouquan Feng , Tianqi Yu , Yongjin Zou , Huan Wen , Shibin Yin . Modulating Metal-Support Interaction Between Pt3Ni and Unsaturated WOx to Selectively Regulate the ORR Performance. Chinese Journal of Structural Chemistry, 2023, 42(10): 100168-100168. doi: 10.1016/j.cjsc.2023.100168
7. [7]
  Lu Dai , Yuxin Ren , Shuang Li , Meidi Wang , Chentao Hu , Ya-Pan Wu , Guangtong Hai , Dong-Sheng Li . Room-temperature synthesis of Co(OH)₂/Mo₂TiC₂T_x hetero-nanosheets with interfacial coupling for enhanced oxygen evolution reaction. Chinese Chemical Letters, 2025, 36(4): 109774-. doi: 10.1016/j.cclet.2024.109774
8. [8]
  Yu Zhang , Weifeng Lin . Electrotunable interfacial friction: A brief review. Chinese Chemical Letters, 2025, 36(4): 110566-. doi: 10.1016/j.cclet.2024.110566
9. [9]
  Yan-Bo Li , Yi Li , Liang Yin . Copper(Ⅰ)-catalyzed diastereodivergent construction of vicinal P-chiral and C-chiral centers facilitated by dual "soft-soft" interaction. Chinese Chemical Letters, 2024, 35(7): 109294-. doi: 10.1016/j.cclet.2023.109294
10. [10]
  Yiyang Shen , Zhen Zhang , Ruyi Liang , Tongbo Wu . Unraveling the interplay of DNAzyme and interfacial factors for enhanced biosensing. Chinese Chemical Letters, 2024, 35(12): 109638-. doi: 10.1016/j.cclet.2024.109638
11. [11]
  Shengdong Sun , Cheng Wang , Shikuo Li . Interfacial channel design on the charge migration for photoelectrochemical applications. Chinese Journal of Structural Chemistry, 2024, 43(12): 100398-100398. doi: 10.1016/j.cjsc.2024.100398
12. [12]
  Jiale Zheng , Mei Chen , Huadong Yuan , Jianmin Luo , Yao Wang , Jianwei Nai , Xinyong Tao , Yujing Liu . Electron-microscopical visualization on the interfacial and crystallographic structures of lithium metal anode. Chinese Chemical Letters, 2024, 35(6): 108812-. doi: 10.1016/j.cclet.2023.108812
13. [13]
  Tianyi Hou , Yunhui Huang , Henghui Xu . Interfacial engineering for advanced solid-state Li-metal batteries. Chinese Journal of Structural Chemistry, 2024, 43(7): 100313-100313. doi: 10.1016/j.cjsc.2024.100313
14. [14]
  Haiying Lu , Weijie Li . The electrolyte solvation and interfacial chemistry for anode-free sodium metal batteries. Chinese Journal of Structural Chemistry, 2024, 43(11): 100334-100334. doi: 10.1016/j.cjsc.2024.100334
15. [15]
  Rui HUANG , Shengjie LIU , Qingyuan WU , Nanfeng ZHENG . Enhanced selectivity of catalytic hydrogenation of halogenated nitroaromatics by interfacial effects. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 201-212. doi: 10.11862/CJIC.20240356
16. [16]
  Jialin Cai , Yizhe Chen , Ruiwen Zhang , Cheng Yuan , Zeyu Jin , Yongting Chen , Shiming Zhang , Jiujun Zhang . Interfacial Pt-N coordination for promoting oxygen reduction reaction. Chinese Chemical Letters, 2025, 36(2): 110255-. doi: 10.1016/j.cclet.2024.110255
17. [17]
  Xu Li , Yue Zhao , Tingli Ma . Improved polymer electrolyte interfacial contact via constructing vertically aligned fillers. Chinese Journal of Structural Chemistry, 2025, 44(2): 100406-100406. doi: 10.1016/j.cjsc.2024.100406
18. [18]
  Yiming Fang , Huimin Gao , Kaiting Cheng , Liang Bai , Zhengtong Li , Yadong Zhao , Xingtao Xu . An overview of photothermal materials for solar-driven interfacial evaporation. Chinese Chemical Letters, 2025, 36(3): 109925-. doi: 10.1016/j.cclet.2024.109925
19. [19]
  Shuxin Liu , Jinjuan Ma , Aiguo Wang , Nan Zheng . Decomposable and sono-enzyme co-triggered poly(sonosensitizers) for precise and hypotoxic sonodynamic therapy. Chinese Chemical Letters, 2025, 36(4): 110032-. doi: 10.1016/j.cclet.2024.110032
20. [20]
  Dong Cheng , Youyou Feng , Bingxi Feng , Ke Wang , Guoxin Song , Gen Wang , Xiaoli Cheng , Yonghui Deng , Jing Wei . Polyphenol-mediated interfacial deposition strategy for supported manganese oxide catalysts with excellent pollutant degradation performance. Chinese Chemical Letters, 2024, 35(5): 108623-. doi: 10.1016/j.cclet.2023.108623

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(712)
HTML views(0)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142