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Citation:
Wei Qu, Si Chen, Shan Ren, Xue-jun Jiang, Ren-xi Zhuo, Xian-zheng Zhang. A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO[J]. Chinese Journal of Polymer Science,
;2013, 31(5): 713-718.
doi:
10.1007/s10118-013-1270-3
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A disulfide bond containing polypeptide, PolyK6-R8, was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK6HC and CR8C. The molar ratio between CHK6HC and CR8C within obtained PolyK6-R8 was 9:1, and PolyK6-R8 could combine DNA compactly when weight ratio reached 5. Through in vitro investigation, it was found that PolyK6-R8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells. Cellular uptake of DNA mediated by PolyK6-R8/DNA complexes was promoted after incubation for 4 h. Moreover, by examining the histological sections of hindlimb ischemia rats through immunohistochemistry, PolyK6-R8/VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation. The results indicated that polypeptide-based PolyK6-R8 is a promising gene vector for the limb ischemia treatment.
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Keywords:
- Gene vector,
- Polypeptide,
- Angiogenesis,
- Limb ischemia
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