Citation: Hong-wei Wang, Lin Yuan, Tie-liang Zhao, He Huang, Hong Chen, Di Wu. ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS[J]. Chinese Journal of Polymer Science, ;2012, 30(6): 893-899. doi: 10.1007/s10118-012-1181-8 shu

ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS

Received Date: 28 March 2012
Revised Date: 18 May 2012

Fund Project: The work was financially supported by the National Natural Science Foundation of China (Nos. 20920102035, 20974086, and 21074083) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

The purpose of this research is to investigate the effects of the variously sulfated chitosans on lysozyme activity and structure. It was shown that the specific enzymatic activity of lysozyme remained almost similar to the native protein after being bound to 6-O-sulfated chitosan (6S-chitosan) and 3,6-O-sulfated chitosan (3,6S-chitosan), but decreased greatly after being bound to 2-N-6-O-sulfated chitosan (2,6S-chitosan). Meanwhile, among these sulfated chitosans, 2,6S-chitosan induced the greatest conformational change in lysozyme as indicated by the fluorescence spectra. These findings demonstrated that when sulfated chitosans of different structures bind to lysozyme, lysozyme undergoes conformational change of different magnitudes, which results in corresponding levels of lysozyme activity. Further study on the interaction of sulfated chitosans with lysozyme by surface plasmon resonance (SPR) suggested that their affinities might be determined by their molecular structures.
- Lysozyme,
- Sulfated chitosan,
- Activity,
- Structure,
- Regioselective modification
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