Citation: Song LI, Jian Xin TANG, Mei Ju JI, Peng HOU, Peng Feng XIAO, Nong Yue HE. A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane[J]. Chinese Chemical Letters, ;2004, 15(7): 837-840. shu

A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane

Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096

Received Date: 13 July 2003

Fund Project: This research is supported by the National Natural Science Foundation of China, Trans-Century Training Programme Foundation for the Talents by the ministry of Education of China and the high-technology program of Jiangsu province (BG2001010).

A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01mol/L NaOH/(H₂O-CH₃OH) mixture medium. The formation of amines (NH₂) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98%.
- Catalyzed hydrolysis,
- in situ synthesis of oligonucleotides,
- coupling efficiency,
- polyamide-6 membrane
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