Citation: Peng ZHANG, Ji Cun REN, Zuo Jun SHEN. A Novel Method for Quantification of Circulating DNA in Serum by Capillary Zone Electrophoresis[J]. Chinese Chemical Letters, ;2004, 15(9): 1091-1094. shu

A Novel Method for Quantification of Circulating DNA in Serum by Capillary Zone Electrophoresis

1.
Department of Chemistry, Shanghai Jiaotong University, Shanghai 200240;
2.
Anhui Provincial Hospital, Hefei 230001

Received Date: 18 August 2003

Fund Project: This work was supported by the National Natural Science Foundation of China (No.20271033), the key project of National Natural Science Foundation of China the Natural (No.20335020), and Science Foundation of Shanghai (No.02ZA14057).

In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients. We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA), satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma.
- Circulating DNA,
- capillary zone electrophoresis,
- quantification,
- serum
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