Citation: Qiu Luan HU, Feng Lin ZHAO, Ke An LI. Spectrophotometric Study on the Interaction between Arsenazo M and Proteins[J]. Chinese Chemical Letters, ;2002, 13(1): 71-72. shu

Spectrophotometric Study on the Interaction between Arsenazo M and Proteins

1.
Department of Chemistry, Peking University, Beijing 100871;
2.
Department of Chemistry, Luo Yang teachers College, He Nan 471022

Received Date: 11 May 2001

Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Arsenazo M itself.The apparent molar absorptivity of the BSA-Arsenazo M complex is 3.21×10⁵ L·mol^-1×cm^-1.The linear ranges for protein determination are wide (at least 0-100 mg/mL).
- Arsenazo M,
- serum proteins,
- spectrophotometry
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