Citation: LI Fumian, WANG Lin, FENG Xinde. STUDIES ON POLYVINYL ALCOHOL CARRIERS Ⅲ. ACTIVE AMIDES AND ESTERS DERIVED FROM POLYVINYL ALCOHOL AND THEIR APPLICATION IN AFFINITY CHROMATOGRAPHY[J]. Chinese Journal of Polymer Science, ;1986, 4(4): 297-302. shu

STUDIES ON POLYVINYL ALCOHOL CARRIERS Ⅲ. ACTIVE AMIDES AND ESTERS DERIVED FROM POLYVINYL ALCOHOL AND THEIR APPLICATION IN AFFINITY CHROMATOGRAPHY

Received Date: 24 July 1984

Polyvinyl alcohol carriers bearing active amide and active ester groups were prepared by acetaliza-tion of granular macro-reticular water-insolub1e PVA with glyoxylic acid or p-formylbenzoic acidand further activation reaction with benzotriazole or N-hydroxysuccinimide using DCCI as con-densation agent. The active amides or active esters obtained were easy to react with amines suchas n-butylamine or proteins under mild condition. Ovomucoid, an inhibitor of trypsin was immo-bilized on the above-obtained reactive PVA carrier to give effective affinity adsorbent for the separation or purification of trypsin.
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